|
Carbon metabolism |
Transcriptional changes along alternative morphogenetic paths of C. cinerea |
Xie et al. 2020
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
sclerotia |
0.1605 |
0.1087 |
0.0627 |
oidia |
0.5369 |
0.1125 |
0.065 |
vegetative_mycelium |
0.451 |
0.0352 |
0.0203 |
primordia |
0.0823 |
0.0734 |
0.0423 |
hyphal_knot |
0.2501 |
0.0412 |
0.0238 |
young_fruiting_body |
0.133 |
0.1154 |
0.0666 |
|
|
Complex carbon sources 1 (long incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control - glucose |
0.5536 |
0.3853 |
0.2225 |
Wheat bran |
0.761 |
0.2729 |
0.1575 |
Horse manure |
0.1869 |
0.1619 |
0.0935 |
Oak leaves |
0.5151 |
0.296 |
0.1709 |
Hay |
0.5099 |
0.2841 |
0.164 |
Corn stalk |
0.48 |
0.2084 |
0.1203 |
Pectine |
0.5345 |
0.1301 |
0.0751 |
Microcrystalline cellulose |
0.2677 |
0.0703 |
0.0406 |
Xylose |
0.3892 |
0.3796 |
0.2192 |
Cellobiose |
0.6123 |
0.2344 |
0.1353 |
Lignin |
0.4181 |
0.2942 |
0.1698 |
Apple peels |
0.3558 |
0.095 |
0.0549 |
|
|
Coprinopsis challenged with bacteria |
Transcriptional response to Bacillus subtilis and Escherichia coli. |
Kombrink et al. 2018
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
0.2359 |
0.0703 |
0.0406 |
Bsubtilis |
0.3026 |
0.1972 |
0.1139 |
Ecoli |
0.505 |
0.1905 |
0.11 |
|
|
Early development 1 |
Time series expression profiling of basidiospore and oidium germination |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
BS 0h |
0.054 |
0.0472 |
0.0273 |
BS 4h |
0.0763 |
0.099 |
0.0572 |
BS 8h |
0.0552 |
0.0576 |
0.0333 |
BS 12h |
0.131 |
0.0487 |
0.0281 |
Oidia 0 h |
0.1601 |
0.1476 |
0.0852 |
Oidia 18 h |
0.5052 |
0.1344 |
0.0776 |
Sclerotia |
0.3278 |
0.2937 |
0.1695 |
|
|
Early development 2 |
Time series expression profiling of mycelial growth, light induction and fruiting body initiation. |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
DM 12h |
0.2339 |
0.0432 |
0.025 |
DM 36h |
0.0677 |
0.0626 |
0.0361 |
DM 60h |
0.0976 |
0.0502 |
0.029 |
DM 84h |
0.2748 |
0.0429 |
0.0248 |
DM 108h |
0.3137 |
0.1037 |
0.0599 |
DM 132h |
0.3907 |
0.1223 |
0.0706 |
DM 156hAE |
0.154 |
0.1334 |
0.077 |
ELI1hAE |
0.4395 |
0.1334 |
0.077 |
ELI2hAE |
0.39 |
0.4052 |
0.234 |
LI2hAE |
0.444 |
0.5128 |
0.296 |
LI6hAE |
0.6437 |
0.6681 |
0.3857 |
LI12hAE |
0.5597 |
0.2828 |
0.1633 |
LI18hAE |
0.7163 |
0.5728 |
0.3307 |
LI 24hAE |
0.5182 |
0.5362 |
0.3095 |
DM 156hAM |
0.5722 |
0.3262 |
0.1884 |
ELI1hAM |
0.5384 |
0.2337 |
0.1349 |
ELI2hAM |
0.2048 |
0.1506 |
0.0869 |
LI2hAM |
0.4819 |
0.6107 |
0.3526 |
LI6hAM |
0.4265 |
0.1542 |
0.089 |
LI12hAM |
0.9742 |
0.8596 |
0.4963 |
LI18hAM |
2.3498 |
1.6684 |
0.9632 |
LI 24hAM |
0.5139 |
0.3236 |
0.1868 |
LI 24hHK |
2.293 |
1.6613 |
0.9592 |
L/D 6h |
2.6866 |
1.3502 |
0.7796 |
L/D 12h |
2.169 |
1.2993 |
0.7502 |
L/D 18h |
1.4281 |
1.0401 |
0.6005 |
L/D 24h |
1.5987 |
0.3363 |
0.1941 |
|
|
Fruiting body development |
RNA-Seq analysis of fruiting body development |
Krizsan et al. 2019
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Vegetative mycelium |
2.1253 |
1.6841 |
0.9723 |
Hyphal knots |
0.8657 |
0.5544 |
0.3201 |
Primordium 1 |
1.2285 |
0.7746 |
0.4472 |
Primordium 2 |
1.7132 |
0.3129 |
0.1806 |
Young Fruiting body cap |
7.065 |
3.7419 |
2.1604 |
Younf fruiting body gill |
0.0564 |
0.0472 |
0.0273 |
Young fruiting body stipe |
0.4223 |
0.252 |
0.1455 |
Mature fruiting body cap_gill |
5.1267 |
1.7341 |
1.0012 |
Mature fruiting body stipe |
4.5554 |
0.4957 |
0.2862 |
|
|
Nitrogen sources (12h incubation) |
Expression profiling on diverse nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Complete medium - control |
0.5342 |
0.2096 |
0.121 |
NH4NO3 |
0.507 |
0.1457 |
0.0841 |
Proline |
0.4646 |
0.1608 |
0.0928 |
Tryptophan |
0.2283 |
0.138 |
0.0797 |
Isoleucin |
0.2321 |
0.0577 |
0.0333 |
Arginine |
0.2127 |
0.1186 |
0.0685 |
Metionine |
0.4004 |
0.1098 |
0.0634 |
NaNO3 |
0.3706 |
0.069 |
0.0399 |
|
|
Plant biomass (12h incubation) |
Expression profiling on diverse plant biomasses as carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
0.5342 |
0.2096 |
0.121 |
Citrus peel |
0.7912 |
0.379 |
0.2188 |
Poplar leaves |
0.306 |
0.1478 |
0.0853 |
Typha leaves |
0.5784 |
0.2574 |
0.1486 |
Overwintered Salix leaves |
0.4926 |
0.1099 |
0.0635 |
Reed flowering |
0.5273 |
0.1173 |
0.0677 |
Tigernut |
0.9807 |
0.1381 |
0.0798 |
Energy cane |
0.5777 |
0.3201 |
0.1848 |
Guar gum |
0.6681 |
0.624 |
0.3603 |
Apple peels |
0.6513 |
0.3299 |
0.1905 |
Cellobiose |
0.299 |
0.1846 |
0.1066 |
Corn stalk |
0.3518 |
0.1412 |
0.0815 |
Horse manure |
0.3727 |
0.0872 |
0.0503 |
Lignin |
0.7634 |
0.3552 |
0.2051 |
Microcrystalline cellulose |
0.6438 |
0.1467 |
0.0847 |
Oak leaves |
0.3067 |
0.1088 |
0.0628 |
Pectin esterified |
0.6864 |
0.0501 |
0.0289 |
Poplar sawdust |
0.3852 |
0.0368 |
0.0213 |
Wheat bran |
0.6969 |
0.2627 |
0.1517 |
Chlamydomonas reinhardtii |
0.4858 |
0.1076 |
0.0621 |
Vertatryl alcohol |
0.0563 |
0.059 |
0.034 |
Furfural |
0.3352 |
0.1944 |
0.1122 |
Autoclaved mycelium |
0.3042 |
0.0767 |
0.0443 |
|
|
Simple and complex carbon and nitrogen sources |
Expression profiling on diverse complex carbon and nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
1.3885 |
0.4173 |
0.241 |
Ribose |
0.7175 |
0.339 |
0.1957 |
Mannose |
1.0012 |
0.2839 |
0.2007 |
Fructose |
1.0125 |
0.595 |
0.3435 |
Arabinose |
0.4749 |
0.1141 |
0.0659 |
Xylose |
0.9868 |
0.3962 |
0.2287 |
Galacturonic acid |
0.6331 |
0.2467 |
0.1424 |
Rhamnogalacturonan |
1.6925 |
0.8108 |
0.4681 |
Pectin esterified |
1.3446 |
0.2818 |
0.1627 |
Polygalacturonic acid |
1.1939 |
0.5882 |
0.3396 |
Sodium acetate |
0.4187 |
0.174 |
0.1004 |
No nitrogen |
1.4465 |
0.351 |
0.2027 |
BSA |
1.459 |
0.2557 |
0.1476 |
Glutamine |
0.9223 |
0.2036 |
0.1176 |
No phosphate |
1.9532 |
0.8664 |
0.5002 |
No carbon |
0.8333 |
0.1808 |
0.1278 |
No sulphur |
0.7796 |
0.2582 |
0.1491 |
|
|
Simple and complex carbon sources (12h incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
0.5342 |
0.2096 |
0.121 |
Amylose |
0.4386 |
0.1833 |
0.1058 |
Fucose |
0.5078 |
0.1354 |
0.0782 |
Galactose |
0.3767 |
0.0069 |
0.004 |
Lactose |
0.3647 |
0.09 |
0.052 |
Maltose |
0.456 |
0.1739 |
0.1004 |
Mannitol |
0.4544 |
0.2135 |
0.1232 |
Rhamnose |
0.3521 |
0.0425 |
0.0245 |
Sorbitol |
0.3168 |
0.1268 |
0.0732 |
Trehalose |
0.483 |
0.0519 |
0.03 |
Glycerol |
0.4353 |
0.1066 |
0.0616 |
Glucuronic acid |
0.3162 |
0.0421 |
0.0243 |
Arabinan |
0.6072 |
0.2321 |
0.134 |
Galactan |
0.6845 |
0.1952 |
0.1127 |
Galactomannan |
0.4139 |
0.2565 |
0.1481 |
Glucan |
0.7644 |
0.1753 |
0.1012 |
Xylan |
0.3223 |
0.0611 |
0.0353 |
Xyloglucan |
0.2875 |
0.0678 |
0.0391 |
Arabinogalactan |
0.2257 |
0.1156 |
0.0667 |
Pectin |
0.7975 |
0.53 |
0.306 |
Rhamnogalacturonan |
1.0108 |
0.4131 |
0.2385 |
Polygalacturonic acid |
0.2937 |
0.0633 |
0.0366 |
Mannan |
0.5006 |
0.1821 |
0.1052 |
Amylopectin |
1.1667 |
0.4262 |
0.2461 |
Inulin |
0.5013 |
0.2518 |
0.1454 |
BSA |
0.5134 |
0.1253 |
0.0723 |
|
|
Starvation induced by water agar transfer |
Expression profiling on starvation induced by transfer to water agar |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
0.0976 |
0.0502 |
0.029 |
2h WAT |
0.2608 |
0.0087 |
0.0051 |
4h WAT |
0.5924 |
0.5575 |
0.3219 |
8h WAT |
0.2405 |
0.3032 |
0.1751 |
16h WAT |
0.7915 |
0.5178 |
0.299 |
24h WAT |
0.4082 |
0.2749 |
0.1587 |
WAT hyphal knot -30h |
0.0146 |
0.0253 |
0.0146 |
WAT hyphal knot -22h |
0.109 |
0.0956 |
0.0552 |
WAT hyphal knot -15h |
0.2952 |
0.291 |
0.168 |
WAT hyphal knot -7.5h |
0.5098 |
0.5342 |
0.3084 |
WAT hyphal knot |
0.1778 |
0.1368 |
0.079 |
WAT hyphal knot +7.5h |
0.268 |
0.0496 |
0.0286 |
|
|
Straw time series |
Time series expression profiling on straw as a carbon source |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
0.939 |
0.4912 |
0.2836 |
Straw 3h |
0.7635 |
0.1987 |
0.1147 |
Straw 6h |
1.0936 |
0.1362 |
0.0786 |
Straw 12h |
0.7491 |
0.1763 |
0.1018 |
Straw 24h |
0.495 |
0.1366 |
0.0789 |
|
|
Stress conditions 1 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
0.4546 |
0.4763 |
0.275 |
Frost (-20C, 30min) |
0.5374 |
0.0733 |
0.0423 |
Heat shock (47C, 2h) |
0.1661 |
0.1007 |
0.0581 |
High CO2 |
0.8047 |
0.4489 |
0.2592 |
Drought (4% agar, 12h) |
1.3385 |
0.5053 |
0.2917 |
Oxidative (1mM H2O2, 3h) |
0.5062 |
0.5706 |
0.3294 |
Acidic (pH4, 3h) |
0.295 |
0.262 |
0.1513 |
Alkaline (pH9, 3h) |
0.3485 |
0.299 |
0.1726 |
Osmotic (sorbitol 2h) |
0.5137 |
0.4449 |
0.2569 |
CongoRed (3h) |
0.3477 |
0.2313 |
0.1336 |
Cobalt chloride control |
0.9186 |
0.3664 |
0.2115 |
Cobalt chloride 2mM |
0.603 |
0.1358 |
0.0784 |
|
|
Stress conditions 2 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
0.1189 |
0.2059 |
0.1189 |
Cold stimulation |
0.4291 |
0.3985 |
0.2301 |
Scratched surface |
0.0644 |
0.0803 |
0.0464 |
Ca2+, 200mM, 24h |
1.7518 |
0.715 |
0.4128 |
Cu2+, 2mM, 6h |
3.9944 |
5.7289 |
3.3076 |
Voriconazole |
0.3413 |
0.3101 |
0.1791 |
Trichoderma interaction, early |
0.2755 |
0.2404 |
0.1388 |
Trichoderma interaction 60h |
0 |
0 |
0 |
Cobalt chloride 2mM repeat |
0.1335 |
0.1762 |
0.1017 |
Hypoxia |
0.4862 |
0.323 |
0.1865 |
Hypoxia control |
0.2932 |
0.2231 |
0.1288 |
Protoplastation control |
1.1573 |
0.3304 |
0.2336 |
Protoplastation 3.5h |
0.9388 |
0.3172 |
0.2243 |
Protoplastation 1.5h |
2.3404 |
0.799 |
0.5649 |
|