|
Carbon metabolism |
Transcriptional changes along alternative morphogenetic paths of C. cinerea |
Xie et al. 2020
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
sclerotia |
0.1934 |
0.0947 |
0.0547 |
oidia |
0.5488 |
0.0958 |
0.0553 |
vegetative_mycelium |
0.4652 |
0.052 |
0.03 |
primordia |
0.0823 |
0.0734 |
0.0423 |
hyphal_knot |
0.2734 |
0.043 |
0.0248 |
young_fruiting_body |
0.133 |
0.1154 |
0.0666 |
|
|
Complex carbon sources 1 (long incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control - glucose |
0.5536 |
0.3853 |
0.2225 |
Wheat bran |
0.761 |
0.2729 |
0.1575 |
Horse manure |
0.1869 |
0.1619 |
0.0935 |
Oak leaves |
0.5151 |
0.296 |
0.1709 |
Hay |
0.5099 |
0.2841 |
0.164 |
Corn stalk |
0.48 |
0.2084 |
0.1203 |
Pectine |
0.5465 |
0.111 |
0.0641 |
Microcrystalline cellulose |
0.2677 |
0.0703 |
0.0406 |
Xylose |
0.3892 |
0.3796 |
0.2192 |
Cellobiose |
0.6123 |
0.2344 |
0.1353 |
Lignin |
0.4181 |
0.2942 |
0.1698 |
Apple peels |
0.3673 |
0.0886 |
0.0512 |
|
|
Coprinopsis challenged with bacteria |
Transcriptional response to Bacillus subtilis and Escherichia coli. |
Kombrink et al. 2018
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
0.2359 |
0.0703 |
0.0406 |
Bsubtilis |
0.3026 |
0.1972 |
0.1139 |
Ecoli |
0.505 |
0.1905 |
0.11 |
|
|
Early development 1 |
Time series expression profiling of basidiospore and oidium germination |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
BS 0h |
0.054 |
0.0472 |
0.0273 |
BS 4h |
0.0763 |
0.099 |
0.0572 |
BS 8h |
0.0552 |
0.0576 |
0.0333 |
BS 12h |
0.131 |
0.0487 |
0.0281 |
Oidia 0 h |
0.1601 |
0.1476 |
0.0852 |
Oidia 18 h |
0.5052 |
0.1344 |
0.0776 |
Sclerotia |
0.3278 |
0.2937 |
0.1695 |
|
|
Early development 2 |
Time series expression profiling of mycelial growth, light induction and fruiting body initiation. |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
DM 12h |
0.2339 |
0.0432 |
0.025 |
DM 36h |
0.0677 |
0.0626 |
0.0361 |
DM 60h |
0.0976 |
0.0502 |
0.029 |
DM 84h |
0.2748 |
0.0429 |
0.0248 |
DM 108h |
0.3137 |
0.1037 |
0.0599 |
DM 132h |
0.3907 |
0.1223 |
0.0706 |
DM 156hAE |
0.154 |
0.1334 |
0.077 |
ELI1hAE |
0.3787 |
0.0321 |
0.0185 |
ELI2hAE |
0.39 |
0.4052 |
0.234 |
LI2hAE |
0.444 |
0.5128 |
0.296 |
LI6hAE |
0.5948 |
0.586 |
0.3383 |
LI12hAE |
0.5597 |
0.2828 |
0.1633 |
LI18hAE |
0.5802 |
0.3373 |
0.1948 |
LI 24hAE |
0.4954 |
0.5444 |
0.3143 |
DM 156hAM |
0.5695 |
0.2658 |
0.1535 |
ELI1hAM |
0.5384 |
0.2337 |
0.1349 |
ELI2hAM |
0.2048 |
0.1506 |
0.0869 |
LI2hAM |
0.4492 |
0.6432 |
0.3713 |
LI6hAM |
0.4494 |
0.1599 |
0.0923 |
LI12hAM |
0.929 |
0.8051 |
0.4648 |
LI18hAM |
2.4898 |
1.8473 |
1.0665 |
LI 24hAM |
0.5447 |
0.376 |
0.2171 |
LI 24hHK |
2.2639 |
1.6455 |
0.95 |
L/D 6h |
2.6866 |
1.3502 |
0.7796 |
L/D 12h |
2.169 |
1.2993 |
0.7502 |
L/D 18h |
1.4034 |
1.019 |
0.5883 |
L/D 24h |
1.5439 |
0.2934 |
0.1694 |
|
|
Fruiting body development |
RNA-Seq analysis of fruiting body development |
Krizsan et al. 2019
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Vegetative mycelium |
2.2281 |
1.722 |
0.9942 |
Hyphal knots |
0.8777 |
0.5628 |
0.325 |
Primordium 1 |
1.2761 |
0.7573 |
0.4372 |
Primordium 2 |
1.7789 |
0.288 |
0.1663 |
Young Fruiting body cap |
7.2878 |
3.8639 |
2.2308 |
Younf fruiting body gill |
0.0564 |
0.0472 |
0.0273 |
Young fruiting body stipe |
0.4331 |
0.2427 |
0.1401 |
Mature fruiting body cap_gill |
5.2625 |
1.7333 |
1.0007 |
Mature fruiting body stipe |
4.705 |
0.5411 |
0.3124 |
|
|
Nitrogen sources (12h incubation) |
Expression profiling on diverse nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Complete medium - control |
0.5625 |
0.1862 |
0.1075 |
NH4NO3 |
0.507 |
0.1457 |
0.0841 |
Proline |
0.4646 |
0.1608 |
0.0928 |
Tryptophan |
0.236 |
0.1482 |
0.0855 |
Isoleucin |
0.2321 |
0.0577 |
0.0333 |
Arginine |
0.2127 |
0.1186 |
0.0685 |
Metionine |
0.4004 |
0.1098 |
0.0634 |
NaNO3 |
0.3789 |
0.0755 |
0.0436 |
|
|
Plant biomass (12h incubation) |
Expression profiling on diverse plant biomasses as carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
0.5625 |
0.1862 |
0.1075 |
Citrus peel |
0.7912 |
0.379 |
0.2188 |
Poplar leaves |
0.306 |
0.1478 |
0.0853 |
Typha leaves |
0.5993 |
0.2763 |
0.1595 |
Overwintered Salix leaves |
0.5444 |
0.1177 |
0.0679 |
Reed flowering |
0.5525 |
0.1403 |
0.081 |
Tigernut |
1.0148 |
0.1283 |
0.0741 |
Energy cane |
0.5777 |
0.3201 |
0.1848 |
Guar gum |
0.6681 |
0.624 |
0.3603 |
Apple peels |
0.6596 |
0.3444 |
0.1988 |
Cellobiose |
0.299 |
0.1846 |
0.1066 |
Corn stalk |
0.3518 |
0.1412 |
0.0815 |
Horse manure |
0.3727 |
0.0872 |
0.0503 |
Lignin |
0.8058 |
0.4176 |
0.2411 |
Microcrystalline cellulose |
0.6438 |
0.1467 |
0.0847 |
Oak leaves |
0.3067 |
0.1088 |
0.0628 |
Pectin esterified |
0.6864 |
0.0501 |
0.0289 |
Poplar sawdust |
0.3916 |
0.028 |
0.0161 |
Wheat bran |
0.7151 |
0.2823 |
0.163 |
Chlamydomonas reinhardtii |
0.4858 |
0.1076 |
0.0621 |
Vertatryl alcohol |
0.0648 |
0.0597 |
0.0345 |
Furfural |
0.3352 |
0.1944 |
0.1122 |
Autoclaved mycelium |
0.3042 |
0.0767 |
0.0443 |
|
|
Simple and complex carbon and nitrogen sources |
Expression profiling on diverse complex carbon and nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
1.3885 |
0.4173 |
0.241 |
Ribose |
0.7175 |
0.339 |
0.1957 |
Mannose |
1.0012 |
0.2839 |
0.2007 |
Fructose |
1.0125 |
0.595 |
0.3435 |
Arabinose |
0.4749 |
0.1141 |
0.0659 |
Xylose |
0.9707 |
0.3697 |
0.2135 |
Galacturonic acid |
0.6331 |
0.2467 |
0.1424 |
Rhamnogalacturonan |
1.6925 |
0.8108 |
0.4681 |
Pectin esterified |
1.3446 |
0.2818 |
0.1627 |
Polygalacturonic acid |
1.2299 |
0.5563 |
0.3212 |
Sodium acetate |
0.4341 |
0.1986 |
0.1146 |
No nitrogen |
1.4565 |
0.377 |
0.2177 |
BSA |
1.459 |
0.2557 |
0.1476 |
Glutamine |
0.9484 |
0.2195 |
0.1267 |
No phosphate |
1.9698 |
0.8572 |
0.4949 |
No carbon |
0.8479 |
0.2014 |
0.1424 |
No sulphur |
0.7886 |
0.2697 |
0.1557 |
|
|
Simple and complex carbon sources (12h incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
0.5625 |
0.1862 |
0.1075 |
Amylose |
0.4628 |
0.2032 |
0.1173 |
Fucose |
0.5309 |
0.123 |
0.071 |
Galactose |
0.3767 |
0.0069 |
0.004 |
Lactose |
0.3647 |
0.09 |
0.052 |
Maltose |
0.5005 |
0.2204 |
0.1272 |
Mannitol |
0.4544 |
0.2135 |
0.1232 |
Rhamnose |
0.3696 |
0.0705 |
0.0407 |
Sorbitol |
0.3168 |
0.1268 |
0.0732 |
Trehalose |
0.5118 |
0.0783 |
0.0452 |
Glycerol |
0.4353 |
0.1066 |
0.0616 |
Glucuronic acid |
0.3162 |
0.0421 |
0.0243 |
Arabinan |
0.6528 |
0.26 |
0.1501 |
Galactan |
0.6661 |
0.1811 |
0.1045 |
Galactomannan |
0.4757 |
0.1978 |
0.1142 |
Glucan |
0.7742 |
0.1664 |
0.096 |
Xylan |
0.3383 |
0.0751 |
0.0434 |
Xyloglucan |
0.2875 |
0.0678 |
0.0391 |
Arabinogalactan |
0.2331 |
0.1285 |
0.0742 |
Pectin |
0.8096 |
0.5229 |
0.3019 |
Rhamnogalacturonan |
1.0254 |
0.3995 |
0.2307 |
Polygalacturonic acid |
0.2937 |
0.0633 |
0.0366 |
Mannan |
0.5006 |
0.1821 |
0.1052 |
Amylopectin |
1.1765 |
0.4261 |
0.246 |
Inulin |
0.5196 |
0.228 |
0.1316 |
BSA |
0.5271 |
0.1019 |
0.0588 |
|
|
Starvation induced by water agar transfer |
Expression profiling on starvation induced by transfer to water agar |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
0.0976 |
0.0502 |
0.029 |
2h WAT |
0.2608 |
0.0087 |
0.0051 |
4h WAT |
0.5512 |
0.4939 |
0.2852 |
8h WAT |
0.2405 |
0.3032 |
0.1751 |
16h WAT |
0.6431 |
0.6845 |
0.3952 |
24h WAT |
0.4082 |
0.2749 |
0.1587 |
WAT hyphal knot -30h |
0.0146 |
0.0253 |
0.0146 |
WAT hyphal knot -22h |
0.109 |
0.0956 |
0.0552 |
WAT hyphal knot -15h |
0.2952 |
0.291 |
0.168 |
WAT hyphal knot -7.5h |
0.5098 |
0.5342 |
0.3084 |
WAT hyphal knot |
0.1354 |
0.1748 |
0.1009 |
WAT hyphal knot +7.5h |
0.268 |
0.0496 |
0.0286 |
|
|
Straw time series |
Time series expression profiling on straw as a carbon source |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
0.9469 |
0.4859 |
0.2805 |
Straw 3h |
0.785 |
0.1703 |
0.0983 |
Straw 6h |
1.0936 |
0.1362 |
0.0786 |
Straw 12h |
0.7672 |
0.1665 |
0.0961 |
Straw 24h |
0.5077 |
0.1485 |
0.0857 |
|
|
Stress conditions 1 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
0.4546 |
0.4763 |
0.275 |
Frost (-20C, 30min) |
0.5374 |
0.0733 |
0.0423 |
Heat shock (47C, 2h) |
0.1661 |
0.1007 |
0.0581 |
High CO2 |
0.8047 |
0.4489 |
0.2592 |
Drought (4% agar, 12h) |
1.3385 |
0.5053 |
0.2917 |
Oxidative (1mM H2O2, 3h) |
0.5062 |
0.5706 |
0.3294 |
Acidic (pH4, 3h) |
0.295 |
0.262 |
0.1513 |
Alkaline (pH9, 3h) |
0.3485 |
0.299 |
0.1726 |
Osmotic (sorbitol 2h) |
0.5137 |
0.4449 |
0.2569 |
CongoRed (3h) |
0.3477 |
0.2313 |
0.1336 |
Cobalt chloride control |
0.887 |
0.3138 |
0.1812 |
Cobalt chloride 2mM |
0.603 |
0.1358 |
0.0784 |
|
|
Stress conditions 2 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
0.1189 |
0.2059 |
0.1189 |
Cold stimulation |
0.4291 |
0.3985 |
0.2301 |
Scratched surface |
0.0644 |
0.0803 |
0.0464 |
Ca2+, 200mM, 24h |
1.599 |
0.8231 |
0.4752 |
Cu2+, 2mM, 6h |
4.1132 |
5.9343 |
3.4262 |
Voriconazole |
0.3413 |
0.3101 |
0.1791 |
Trichoderma interaction, early |
0.2755 |
0.2404 |
0.1388 |
Trichoderma interaction 60h |
0 |
0 |
0 |
Cobalt chloride 2mM repeat |
0.0891 |
0.1017 |
0.0587 |
Hypoxia |
0.4862 |
0.323 |
0.1865 |
Hypoxia control |
0.2932 |
0.2231 |
0.1288 |
Protoplastation control |
1.103 |
0.4073 |
0.288 |
Protoplastation 3.5h |
0.9388 |
0.3172 |
0.2243 |
Protoplastation 1.5h |
2.3404 |
0.799 |
0.5649 |
|