CopciAB_108063
Coprinopsis cinerea A43mutB43mut pab1-1 #326

General data

Systematic name CopciAB_108063 Strain Coprinopsis cinerea Okayama 7
Standard name - Synonyms 108063
Uniprot id Functional description Protein tyrosine kinase
Location scaffold_2:2217503..2220051 Strand -
Gene length (nt) 2549 Transcript length (nt) 1694
CDS length (nt) 1566 Protein length (aa) 521

Reciprocal best hits in model fungi

Strain name Gene / Protein name

Orthologs in mushroom models

Strain name Gene / Protein name Pident E-value Bits
Hypsizygus marmoreus strain 51987-8 Hypma_RDB21515 51 1.577E-174 552
Pleurotus eryngii ATCC 90797 Pleery1_1446746 45.5 1.904E-150 482
Pleurotus ostreatus PC15 PleosPC15_2_1099610 43.5 2.211E-146 470
Agrocybe aegerita Agrae_CAA7267978 46.6 3.67E-142 458
Agaricus bisporus var. burnettii JB137-S8 Agabi_varbur_1_108151 35.6 5.881E-109 361
Schizophyllum commune H4-8 Schco3_2484255 42 1.218E-89 305
Agaricus bisporus var bisporus (H97) Agabi_varbisH97_2_194189 49.4 4.67E-85 291

Expression

Name Summary Attribution Assay type
Carbon metabolism Transcriptional changes along alternative morphogenetic paths of C. cinerea Xie et al. 2020 RNA-Seq
Sample Mean cpm SD cpm Se of mean
sclerotia 71.6763 9.5887 5.536
oidia 40.2112 12.1428 7.0106
vegetative_mycelium 38.9987 5.3748 3.1031
primordia 33.6303 4.0956 2.3646
hyphal_knot 70.7806 11.7404 6.7783
young_fruiting_body 20.6705 2.3962 1.3835
Complex carbon sources 1 (long incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control - glucose 36.7272 4.9563 2.8615
Wheat bran 71.0661 17.7573 10.2522
Horse manure 51.0359 7.2993 4.2143
Oak leaves 70.9076 22.8551 13.1954
Hay 47.0039 5.8473 3.376
Corn stalk 50.8926 5.122 2.9572
Pectine 78.8287 6.1967 3.5777
Microcrystalline cellulose 64.0688 6.3097 3.6429
Xylose 48.9092 6.2856 3.629
Cellobiose 40.7228 2.3087 1.3329
Lignin 31.3832 13.5029 7.7959
Apple peels 45.0594 2.2183 1.2807
Coprinopsis challenged with bacteria Transcriptional response to Bacillus subtilis and Escherichia coli. Kombrink et al. 2018 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control 23.3769 2.23 1.2875
Bsubtilis 26.3924 2.2786 1.3156
Ecoli 21.8034 3.2597 1.882
Early development 1 Time series expression profiling of basidiospore and oidium germination Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
BS 0h 3.4305 0.826 0.4769
BS 4h 5.6496 0.7111 0.4105
BS 8h 4.1528 0.618 0.3568
BS 12h 4.0369 0.9214 0.532
Oidia 0 h 6.2641 1.4982 0.865
Oidia 18 h 2.6117 0.4185 0.2416
Sclerotia 24.4994 2.7095 1.5644
Early development 2 Time series expression profiling of mycelial growth, light induction and fruiting body initiation. Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
DM 12h 12.6022 2.7045 1.5615
DM 36h 4.5707 1.1535 0.666
DM 60h 4.7429 0.5849 0.3377
DM 84h 6.9698 3.1265 1.8051
DM 108h 4.0991 0.5177 0.2989
DM 132h 7.1587 3.0725 1.7739
DM 156hAE 7.1497 0.3358 0.1939
ELI1hAE 9.2103 1.0987 0.6343
ELI2hAE 12.6802 8.068 4.658
LI2hAE 11.1075 2.4716 1.427
LI6hAE 15.5127 1.6977 0.9802
LI12hAE 13.1766 6.3601 3.672
LI18hAE 15.7116 4.3693 2.5226
LI 24hAE 8.7846 0.9789 0.5652
DM 156hAM 9.728 1.6782 0.9689
ELI1hAM 12.3496 5.5496 3.2041
ELI2hAM 11.4552 3.9193 2.2628
LI2hAM 7.8799 3.2401 1.8707
LI6hAM 12.1764 3.5814 2.0677
LI12hAM 24.531 5.4712 3.1588
LI18hAM 27.3443 9.9994 5.7731
LI 24hAM 14.9263 2.1095 1.2179
LI 24hHK 23.4221 7.9763 4.6051
L/D 6h 25.6592 11.8209 6.8248
L/D 12h 14.9512 3.3915 1.9581
L/D 18h 12.0261 2.328 1.3441
L/D 24h 8.6368 1.3915 0.8034
Fruiting body development RNA-Seq analysis of fruiting body development Krizsan et al. 2019 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Vegetative mycelium 63.4494 16.3836 9.4591
Hyphal knots 59.4129 29.4945 17.0287
Primordium 1 31.265 4.5804 2.6445
Primordium 2 39.752 8.5186 4.9182
Young Fruiting body cap 27.4686 6.2843 3.6282
Younf fruiting body gill 28.5825 4.1179 2.3774
Young fruiting body stipe 50.7347 13.9236 8.0388
Mature fruiting body cap_gill 19.5563 8.9271 5.154
Mature fruiting body stipe 33.8091 7.2336 4.1763
Nitrogen sources (12h incubation) Expression profiling on diverse nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Complete medium - control 20.4504 7.3034 4.2166
NH4NO3 30.4771 4.9601 2.8637
Proline 29.4646 9.9968 5.7717
Tryptophan 29.9134 12.124 6.9998
Isoleucin 27.4659 0.7381 0.4262
Arginine 31.6418 3.4393 1.9857
Metionine 28.4196 0.8666 0.5003
NaNO3 17.9961 1.6153 0.9326
Plant biomass (12h incubation) Expression profiling on diverse plant biomasses as carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 20.4504 7.3034 4.2166
Citrus peel 13.7767 3.7849 2.1852
Poplar leaves 22.7748 6.0951 3.519
Typha leaves 23.1031 4.1519 2.3971
Overwintered Salix leaves 21.8739 4.4482 2.5681
Reed flowering 17.5997 2.7145 1.5672
Tigernut 17.4208 0.5661 0.3269
Energy cane 28.8767 2.4353 1.406
Guar gum 21.4034 4.0922 2.3626
Apple peels 32.1221 2.9717 1.7157
Cellobiose 27.2321 2.3979 1.3844
Corn stalk 39.7933 5.7311 3.3089
Horse manure 49.6303 21.8935 12.6402
Lignin 20.5046 4.0597 2.3438
Microcrystalline cellulose 33.2343 15.1962 8.7735
Oak leaves 41.4117 1.5658 0.904
Pectin esterified 34.5362 14.9473 8.6298
Poplar sawdust 38.8936 15.1358 8.7386
Wheat bran 45.5583 8.837 5.102
Chlamydomonas reinhardtii 38.385 3.4593 1.9972
Vertatryl alcohol 15.7495 1.6319 0.9422
Furfural 13.0277 1.8815 1.0863
Autoclaved mycelium 26.1392 3.4706 2.0037
Simple and complex carbon and nitrogen sources Expression profiling on diverse complex carbon and nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 51.5774 5.6486 3.2612
Ribose 45.4758 1.8009 1.0397
Mannose 42.076 3.9541 2.796
Fructose 46.7399 5.4053 3.1208
Arabinose 50.638 7.8126 4.5106
Xylose 43.1895 1.4374 0.8299
Galacturonic acid 46.7003 4.2637 2.4616
Rhamnogalacturonan 53.4114 4.3832 2.5306
Pectin esterified 59.4674 5.53 3.1927
Polygalacturonic acid 49.6806 7.3309 4.2325
Sodium acetate 38.1809 1.1928 0.6886
No nitrogen 51.4464 5.7471 3.3181
BSA 54.0568 4.5425 2.6226
Glutamine 55.868 1.9249 1.1113
No phosphate 44.5703 3.4723 2.0048
No carbon 47.5796 2.2404 1.5842
No sulphur 46.865 5.8571 3.3816
Simple and complex carbon sources (12h incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 20.4504 7.3034 4.2166
Amylose 44.6844 6.9373 4.0053
Fucose 40.0379 4.4634 2.5769
Galactose 45.3925 6.1984 3.5786
Lactose 35.4256 10.334 5.9663
Maltose 39.7071 7.8477 4.5309
Mannitol 39.0147 11.0513 6.3804
Rhamnose 32.2025 9.5838 5.5332
Sorbitol 38.4198 1.8244 1.0533
Trehalose 28.0778 8.91 5.1442
Glycerol 30.7853 13.1591 7.5974
Glucuronic acid 40.8593 4.6036 2.6579
Arabinan 22.9574 5.3845 3.1087
Galactan 25.1204 0.371 0.2142
Galactomannan 34.7956 13.3762 7.7228
Glucan 27.016 7.7536 4.4765
Xylan 30.7499 5.3442 3.0855
Xyloglucan 28.3055 11.5816 6.6867
Arabinogalactan 42.3836 5.371 3.1009
Pectin 69.1795 22.6865 13.0981
Rhamnogalacturonan 46.3091 22.4603 12.9674
Polygalacturonic acid 52.8499 19.655 11.3478
Mannan 35.3335 3.342 1.9295
Amylopectin 37.3362 24.0037 13.8586
Inulin 54.2238 10.9075 6.2975
BSA 14.1677 2.1616 1.248
Starvation induced by water agar transfer Expression profiling on starvation induced by transfer to water agar Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Control 4.7429 0.5849 0.3377
2h WAT 15.3325 5.4172 3.1276
4h WAT 14.2793 1.0004 0.5776
8h WAT 15.768 1.4863 0.8581
16h WAT 14.2166 2.6873 1.5515
24h WAT 15.7128 3.6157 2.0875
WAT hyphal knot -30h 7.5718 0.5841 0.3372
WAT hyphal knot -22h 7.6358 1.6375 0.9454
WAT hyphal knot -15h 10.0341 2.7465 1.5857
WAT hyphal knot -7.5h 12.2779 2.2818 1.3174
WAT hyphal knot 11.3046 1.5787 0.9115
WAT hyphal knot +7.5h 15.2162 1.8542 1.0705
Straw time series Time series expression profiling on straw as a carbon source Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 41.5996 17.1529 9.9032
Straw 3h 121.4121 81.7749 47.2128
Straw 6h 100.6818 82.6449 47.7151
Straw 12h 56.6722 18.2457 10.5341
Straw 24h 39.4473 38.5066 22.2318
Stress conditions 1 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 6.3411 2.4172 1.3956
Frost (-20C, 30min) 22.6483 4.824 2.7851
Heat shock (47C, 2h) 5.7949 1.6696 0.964
High CO2 15.0202 4.8681 2.8106
Drought (4% agar, 12h) 22.8174 5.3185 3.0706
Oxidative (1mM H2O2, 3h) 11.1847 1.484 0.8568
Acidic (pH4, 3h) 7.0019 1.8454 1.0654
Alkaline (pH9, 3h) 10.4654 1.6007 0.9241
Osmotic (sorbitol 2h) 19.0318 10.491 6.057
CongoRed (3h) 15.7388 5.2846 3.0511
Cobalt chloride control 30.2399 4.4697 2.5806
Cobalt chloride 2mM 37.5659 4.9944 2.8835
Stress conditions 2 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 16.2339 9.2007 5.312
Cold stimulation 9.6745 2.2439 1.2955
Scratched surface 9.8417 0.8575 0.4951
Ca2+, 200mM, 24h 24.5726 2.2801 1.3164
Cu2+, 2mM, 6h 8.9062 2.239 1.2927
Voriconazole 20.1166 2.9631 1.7107
Trichoderma interaction, early 12.579 0.6892 0.3979
Trichoderma interaction 60h 26.5631 12.1344 7.0058
Cobalt chloride 2mM repeat 6.7511 2.6438 1.5264
Hypoxia 13.8124 2.0517 1.1845
Hypoxia control 17.9471 2.7113 1.5653
Protoplastation control 53.9704 2.5124 1.7765
Protoplastation 3.5h 27.605 2.0847 1.4741
Protoplastation 1.5h 45.9068 5.918 4.1846

General data

Systematic name -
Protein id CopciAB_108063.T0
Description Protein tyrosine kinase

Annotation summary

0 100 200 300 400 500 521

Conserved domains

Analysis Signature accession Signature description InterPro Accession Start End
CDD cd21037 MLKL_NTD - 28 161
Pfam PF07714 Protein tyrosine and serine/threonine kinase IPR001245 260 516

SignalP

Prediction Start End Score
No records

Transmembrane domains

Domain n Start End Length
No records

InterPro

Accession Description
IPR001245 Serine-threonine/tyrosine-protein kinase, catalytic domain
IPR011009 Protein kinase-like domain superfamily
IPR000719 Protein kinase domain
IPR036537 Adaptor protein Cbl, N-terminal domain superfamily

GO

Go id Term Ontology
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP
GO:0005524 ATP binding MF
GO:0007166 cell surface receptor signaling pathway BP

KEGG

KEGG Orthology
No records

EggNOG

COG category Description
T Protein tyrosine kinase

CAZy

Class Family Subfamily
No records

Transcription factor

Group
No records

Conservation of CopciAB_108063 across fungi.

Arrow shows the origin of gene family containing CopciAB_108063.

Protein

Sequence id CopciAB_108063.T0
Sequence >CopciAB_108063.T0
MMAKDLVSGILGAVELGANVAGVPFAAGAALILKEIVDTCDQLRIHKKKARTIRNKCVQFTLLLDDHSRTNIDAE
LLKSIDETRAIFERVYSRMHRYSQMSAWSLFLKMSEIGEGLDECEANVQSAIEMFQFAAHAVGNRNQQEIKETMR
VQHEEMRALLREVLTKPSEAREIIAMQRSGSQVAQQVMQAGQEQLRNELMISNTRSESPTREGRDQEATTQKRRP
GSDIRDALYQFHLQTGTLPAIKILNEEITRRSDHAVAGGTHSDIWEGLWLGRKKVALKGLRNIKADDPRAKLRIE
REIKTWSSLQNEHILPFYGIVTDIGTHIHMVSPWQENGNVLDYLKKQPNVNRLRIIKGAARGLAYLHSMKIVHGN
VKCTNILVMDNGEACICDFGMSKLIEEVTEKAASATLTAAGSARWLAPELIDGTISSPTFATDTYSFAMAVLELL
TGKYPFAERKRDASVIHDVVVMKQTPARPQDPRVCVWLTDELWNLLGGCWHNSAESRPTMAEVTGELDLFE
Length 521

Coding

Sequence id CopciAB_108063.T0
Sequence >CopciAB_108063.T0
ATGATGGCTAAGGACCTCGTCTCTGGAATACTTGGTGCAGTCGAACTTGGGGCTAACGTCGCTGGAGTTCCATTC
GCTGCCGGAGCCGCACTTATACTCAAAGAGATTGTCGATACTTGTGACCAGCTTCGTATTCACAAGAAGAAGGCT
AGAACTATCAGAAACAAATGCGTCCAGTTCACACTCCTGCTCGATGACCACTCCCGGACCAACATCGACGCCGAA
CTGCTAAAGTCAATCGATGAAACCAGGGCGATCTTTGAACGAGTGTACTCCCGGATGCATAGGTATTCACAAATG
AGCGCCTGGTCTCTATTCCTCAAGATGTCCGAGATCGGGGAAGGTCTGGACGAATGCGAGGCGAACGTTCAATCT
GCCATTGAGATGTTCCAGTTCGCCGCCCATGCCGTAGGAAATAGAAACCAGCAAGAAATCAAAGAAACTATGCGG
GTCCAGCATGAAGAAATGAGGGCCCTCCTCCGCGAGGTCCTCACTAAGCCCTCGGAGGCAAGGGAGATCATCGCA
ATGCAGAGGTCAGGGAGCCAAGTCGCACAGCAAGTGATGCAAGCCGGTCAAGAGCAATTGCGCAATGAATTGATG
ATCAGCAACACTCGATCCGAGTCCCCGACAAGAGAAGGAAGGGACCAGGAGGCCACAACCCAGAAAAGGCGCCCT
GGAAGTGACATCCGGGATGCACTTTACCAATTCCACTTGCAAACTGGTACACTACCAGCCATAAAGATCCTCAAC
GAGGAGATCACTAGACGCTCAGATCATGCAGTGGCAGGAGGAACTCACAGTGACATCTGGGAAGGGCTCTGGCTA
GGCCGCAAAAAGGTCGCGCTCAAGGGCCTTAGGAACATAAAGGCTGATGATCCCAGGGCCAAGCTGCGAATCGAA
CGCGAGATAAAGACGTGGTCATCGCTCCAGAACGAGCACATATTACCATTCTATGGGATCGTGACCGATATCGGG
ACACATATACATATGGTTTCTCCATGGCAGGAGAACGGAAACGTTCTAGACTATCTCAAGAAACAACCCAACGTA
AACCGTTTACGCATCATTAAAGGTGCGGCACGAGGCTTAGCCTACCTACACTCAATGAAGATAGTTCACGGAAAT
GTGAAATGCACGAACATCCTTGTAATGGACAACGGGGAGGCGTGTATATGTGATTTTGGGATGTCCAAGCTGATC
GAGGAGGTAACAGAGAAGGCAGCCTCGGCGACGTTGACGGCTGCAGGGAGTGCTCGCTGGTTAGCGCCGGAGCTG
ATTGATGGGACCATTTCATCCCCGACGTTTGCGACGGACACCTACTCCTTCGCCATGGCGGTTCTGGAGTTACTT
ACAGGGAAATACCCATTCGCAGAACGTAAGAGGGATGCGTCTGTAATACACGATGTGGTGGTCATGAAGCAGACG
CCTGCTCGCCCGCAAGATCCGAGGGTTTGTGTGTGGTTGACGGATGAGCTGTGGAACCTCTTGGGAGGGTGCTGG
CACAACTCGGCAGAATCGAGACCCACAATGGCGGAAGTGACAGGAGAATTGGATTTATTCGAGTAG
Length 1566

Transcript

Sequence id CopciAB_108063.T0
Sequence >CopciAB_108063.T0
GCATTGCTGTAGACTCCACCTCGCCGTTGTCGTTCCTTGTCCTCTGCCCCCTCATACATTCTATGATGGCTAAGG
ACCTCGTCTCTGGAATACTTGGTGCAGTCGAACTTGGGGCTAACGTCGCTGGAGTTCCATTCGCTGCCGGAGCCG
CACTTATACTCAAAGAGATTGTCGATACTTGTGACCAGCTTCGTATTCACAAGAAGAAGGCTAGAACTATCAGAA
ACAAATGCGTCCAGTTCACACTCCTGCTCGATGACCACTCCCGGACCAACATCGACGCCGAACTGCTAAAGTCAA
TCGATGAAACCAGGGCGATCTTTGAACGAGTGTACTCCCGGATGCATAGGTATTCACAAATGAGCGCCTGGTCTC
TATTCCTCAAGATGTCCGAGATCGGGGAAGGTCTGGACGAATGCGAGGCGAACGTTCAATCTGCCATTGAGATGT
TCCAGTTCGCCGCCCATGCCGTAGGAAATAGAAACCAGCAAGAAATCAAAGAAACTATGCGGGTCCAGCATGAAG
AAATGAGGGCCCTCCTCCGCGAGGTCCTCACTAAGCCCTCGGAGGCAAGGGAGATCATCGCAATGCAGAGGTCAG
GGAGCCAAGTCGCACAGCAAGTGATGCAAGCCGGTCAAGAGCAATTGCGCAATGAATTGATGATCAGCAACACTC
GATCCGAGTCCCCGACAAGAGAAGGAAGGGACCAGGAGGCCACAACCCAGAAAAGGCGCCCTGGAAGTGACATCC
GGGATGCACTTTACCAATTCCACTTGCAAACTGGTACACTACCAGCCATAAAGATCCTCAACGAGGAGATCACTA
GACGCTCAGATCATGCAGTGGCAGGAGGAACTCACAGTGACATCTGGGAAGGGCTCTGGCTAGGCCGCAAAAAGG
TCGCGCTCAAGGGCCTTAGGAACATAAAGGCTGATGATCCCAGGGCCAAGCTGCGAATCGAACGCGAGATAAAGA
CGTGGTCATCGCTCCAGAACGAGCACATATTACCATTCTATGGGATCGTGACCGATATCGGGACACATATACATA
TGGTTTCTCCATGGCAGGAGAACGGAAACGTTCTAGACTATCTCAAGAAACAACCCAACGTAAACCGTTTACGCA
TCATTAAAGGTGCGGCACGAGGCTTAGCCTACCTACACTCAATGAAGATAGTTCACGGAAATGTGAAATGCACGA
ACATCCTTGTAATGGACAACGGGGAGGCGTGTATATGTGATTTTGGGATGTCCAAGCTGATCGAGGAGGTAACAG
AGAAGGCAGCCTCGGCGACGTTGACGGCTGCAGGGAGTGCTCGCTGGTTAGCGCCGGAGCTGATTGATGGGACCA
TTTCATCCCCGACGTTTGCGACGGACACCTACTCCTTCGCCATGGCGGTTCTGGAGTTACTTACAGGGAAATACC
CATTCGCAGAACGTAAGAGGGATGCGTCTGTAATACACGATGTGGTGGTCATGAAGCAGACGCCTGCTCGCCCGC
AAGATCCGAGGGTTTGTGTGTGGTTGACGGATGAGCTGTGGAACCTCTTGGGAGGGTGCTGGCACAACTCGGCAG
AATCGAGACCCACAATGGCGGAAGTGACAGGAGAATTGGATTTATTCGAGTAGTGATGTATGTATTTTGTAGTAG
GTGTCTCCTGGCATTGACTCAACAACGACGACGGGGTTAACGAG
Length 1694

Gene

Sequence id CopciAB_108063.T0
Sequence >CopciAB_108063.T0
GCATTGCTGTAGACTCCACCTCGCCGTTGTCGTTCCTTGTCCTCTGCCCCCTCATACATTCTATGATGGCTAAGG
ACCTCGTCTCTGGAATACTTGGTGCAGTCGAACTTGGGGCTAACGTCGCTGGAGTTCCATTCGCTGCCGGAGCCG
CACTTATACTCAAAGAGATTGTCGATACTTGTGACCAGCTTCGTATTCACAAGGTGAGTGAAACATACAGCTTGA
CCTCTGTATCTGCTTACATTGACTGTAGAAGAAGGCTAGAACTATCAGAAACAAATGCGTCCAGTTCACACTCCT
GCTCGATGACCACTCCCGGACCAACATCGACGCCGAACTGCTAAAGTCAATCGATGAAACCAGGGCGTCAGTTCC
TTTCCATTAATAGACATGGAATTACTCTCTTATGCTTCTTTCATTAGGATCTTTGAACGAGTGTACTCCCGGATG
CATAGGTATTCACAAATGAGCGCCTGGTCTCTATTCCGCAAGTCATGCTTCCCCGTCACCCTATCACATTCCCTT
TCTTACGCACAATCTCAGTCAAGATGTCCGAGATCGGGGAAGGTCTGGACGAATGCGAGGCGAACGTTCAATCTG
CCATTGAGATGTTCCAGGTGGGTGCTTTATCCGTGCCACTTAGTGAGGTAACAACTTATCGAGTGGGACAGTTCG
CGTAAGGCCCTGCTCTGCCTCTTGGCCCATGTCCCAATTCATTCACAGACACCTTCAGCGCCCATGCCGTAGGAA
ATAGAAACCAGCAAGAAATCAAAGAAACTATGCGGGTCCAGCATGAAGAAATGAGGGCCCTCCTCCGCGAGGTCC
TCACTAAGCCCTCGGAGGCAAGGGAGATCATCGCAATGCAGAGGTCAGGGAGCCAAGTCGCACAGCAAGTGATGC
AAGCCGGTCAAGAGGTCTGCTTTCTCCTTGTTCAGTCTCTTCACCTACGTCATTGATATTTCCTTGCGGGCATAG
CAATTGCGCAATGAATTGATGATCAGCAACACTCGATCCGAGTCCCCGACAAGAGAAGGAAGGGACCAGGAGGCC
ACAACCCAGAAAAGGCGCCCTGGAAGTGACATCCGGGATGCACTTTACCAATTCCACTTGCAAACTGGTACACTA
CCAGCCATAAAGATCCTCAACGAGGAGATCACTAGACGCTCAGATCATGCAGTGGCAGGAGGAACTCACAGTGAC
ATCTGGGAAGGGCTCTGGCTAGGCCGCAAAAAGGTACTTCAAGCGAGTAGGGTATCCTATAATCTTTCAGAAACT
CACGGACCCTGTTTCACAGGTCGCGCTCAAGGGCCTTAGGAACATAAAGGCTGATGATCCCAGGGCCAAGCTGGC
AAGTTGACGATCGATTGGACACCTAACCTTCCGTTCTGACTGGGGAATCCAAAAACAGCGAATCGAACGCGAGAT
AAAGACGTGGTCATCGCTCCAGAACGAGCACATATTACCATTCTATGGGATCGTGACCGATATCGGGACACATAT
ACATATGGTATGTCATGGAAAGAACCACCCTAAAGACATTGGCTAAACATATCAGAAAGGTTTCTCCATGGCAGG
AGAACGGAAACGTTCTAGAGTAGGCTTCATATGTCATACAAGCGTCACAAAGGGTTGGTGAACTGACACCTGCCC
AAGCTATCTCAAGAAACAACCCAACGTAAACCGTTTACGCATCGTGAGTGGCCACGTCCCTGTCCTCGTTCCCTC
CTATCGGGCTTACCTGTTTCTCTTCAGATTAAAGGTGCGGCACGAGGCTTAGCCTACCTACACTCAATGAAGATA
GTTCACGGAAATGGTGCGACGATTTCTAATTTTACACCGATAAACTTACATGTGGATTGATGATCGATTCGTGGC
TGGTGCGGTGTGGTTCCACCAGTGAAATGCGTACGTGATTCTTTCCCTTATTGGCCTTGTTTGAAACGTTCAGCT
CACAAAGGTGTGAATGAAACTCTCCTCATACAGACGAACATCCTTGTAATGGACAACGTAAGGACGGGATTCTTT
GAGGTTCTTCGGAACTTGGCGCTGACTATCAGGATGGGTCTCACGTACAGGGGGAGGCGTGTATATGTGATTTTG
GGATGTCCAAGCTGATCGAGGAGGTAACAGAGAAGGCAGCCTCGGCGACGTTGACGGCTGCAGGGAGTGCTCGCT
GGTTAGCGCCGGAGCTGATTGATGGGACCATTTCATCCCCGACGTTTGCGACGGACACCTACTCCTTCGCCATGG
CGGTTCTGGAGTTACTTACAGGGAAATACCCATTCGCAGAACGTAAGAGGGATGCGTCTGTAATACACGATGTGG
TGGTCATGAAGCAGACGCCTGCTCGCCCGCAAGATCCGAGGGTTTGTGTGTGGTTGACGGATGAGCTGTGGAACC
TCTTGGGAGGGTGCTGGCACAACTCGGCAGAATCGAGACCCACAATGGCGGAAGTGACAGGAGAATTGGATTTAT
TCGAGTAGTGATGTATGTATTTTGTAGTAGGTGTCTCCTGGCATTGACTCAACAACGACGACGGGGTTAACGAG
Length 2549