CopciAB_359285
Coprinopsis cinerea A43mutB43mut pab1-1 #326

General data

Systematic name CopciAB_359285 Strain Coprinopsis cinerea Okayama 7
Standard name - Synonyms 359285
Uniprot id Functional description Belongs to the glycosyl hydrolase 5 (cellulase A) family
Location scaffold_4:1317425..1319957 Strand +
Gene length (nt) 2533 Transcript length (nt) 1984
CDS length (nt) 1332 Protein length (aa) 443

Reciprocal best hits in model fungi

Strain name Gene / Protein name
Neurospora crassa NCU08412
Aspergillus niger manA
Aspergillus nidulans AN9276_manF

Orthologs in mushroom models

Strain name Gene / Protein name Pident E-value Bits
Agrocybe aegerita Agrae_CAA7263174 66.5 7.589E-208 644
Hypsizygus marmoreus strain 51987-8 Hypma_RDB16956 67.4 4.208E-207 642
Pleurotus ostreatus PC9 PleosPC9_1_52090 64.4 1.133E-200 623
Pleurotus ostreatus PC15 PleosPC15_2_1041600 62.6 3.658E-196 610
Agaricus bisporus var. burnettii JB137-S8 Agabi_varbur_1_112644 62.9 7.304E-196 609
Agaricus bisporus var bisporus (H97) Agabi_varbisH97_2_192609 62.9 1.555E-195 608
Pleurotus eryngii ATCC 90797 Pleery1_1491814 62.6 4.699E-195 607
Grifola frondosa Grifr_OBZ68183 60.8 8.634E-187 583
Ganoderma sp. 10597 SS1 v1.0 Gansp1_116075 61.1 4.253E-186 581
Flammulina velutipes Flave_chr07AA00834 61.2 7.22E-180 563
Lentinula edodes B17 Lened_B_1_1_6094 59.9 3.044E-179 561
Lentinula edodes NBRC 111202 Lenedo1_1177261 60.1 3.847E-179 561
Lentinula edodes W1-26 v1.0 Lentinedodes1_22615 60.1 3.818E-179 561
Schizophyllum commune H4-8 Schco3_2635181 68.9 3.038E-174 547
Auricularia subglabra Aurde3_1_1148144 55.1 9.405E-165 520

Expression

Name Summary Attribution Assay type
Carbon metabolism Transcriptional changes along alternative morphogenetic paths of C. cinerea Xie et al. 2020 RNA-Seq
Sample Mean cpm SD cpm Se of mean
sclerotia 31.5262 1.4549 0.84
oidia 19.8255 0.7459 0.4306
vegetative_mycelium 18.874 1.5063 0.8696
primordia 9.0946 0.5523 0.3189
hyphal_knot 21.9814 2.1925 1.2658
young_fruiting_body 2.545 1.166 0.6732
Complex carbon sources 1 (long incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control - glucose 16.2408 3.3339 1.9248
Wheat bran 16.6034 5.3679 3.0992
Horse manure 150.1606 66.0745 38.1482
Oak leaves 236.4596 123.3861 71.237
Hay 411.6618 104.8953 60.5613
Corn stalk 388.6612 62.4263 36.0418
Pectine 21.0109 0.5884 0.3397
Microcrystalline cellulose 71.7069 9.9906 5.7681
Xylose 16.429 0.8735 0.5043
Cellobiose 12.1088 1.3156 0.7596
Lignin 36.978 17.571 10.1446
Apple peels 16.4381 3.5544 2.0521
Coprinopsis challenged with bacteria Transcriptional response to Bacillus subtilis and Escherichia coli. Kombrink et al. 2018 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control 10.3735 1.4647 0.8456
Bsubtilis 11.849 1.1946 0.6897
Ecoli 14.2316 1.19 0.6871
Early development 1 Time series expression profiling of basidiospore and oidium germination Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
BS 0h 7.1058 0.4779 0.2759
BS 4h 3.7975 1.3218 0.7631
BS 8h 3.6887 0.6908 0.3988
BS 12h 4.7157 0.4552 0.2628
Oidia 0 h 5.9777 1.1535 0.6659
Oidia 18 h 3.1937 1.2566 0.7255
Sclerotia 7.3233 3.1058 1.7931
Early development 2 Time series expression profiling of mycelial growth, light induction and fruiting body initiation. Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
DM 12h 3.0639 1.0332 0.5965
DM 36h 3.0899 0.4764 0.275
DM 60h 2.8818 1.3056 0.7538
DM 84h 1.8612 0.425 0.2454
DM 108h 2.8087 0.9429 0.5444
DM 132h 2.8889 1.41 0.8141
DM 156hAE 2.3923 0.4921 0.2841
ELI1hAE 2.6732 0.1241 0.0716
ELI2hAE 2.917 0.3905 0.2254
LI2hAE 2.3692 0.9379 0.5415
LI6hAE 2.8442 1.3946 0.8052
LI12hAE 2.0484 0.4964 0.2866
LI18hAE 2.6406 1.4181 0.8188
LI 24hAE 3.2401 0.684 0.3949
DM 156hAM 5.352 2.2593 1.3044
ELI1hAM 2.4046 0.6926 0.3999
ELI2hAM 2.9106 0.5894 0.3403
LI2hAM 2.4221 0.4801 0.2772
LI6hAM 1.5366 0.8503 0.4909
LI12hAM 2.3303 0.8361 0.4827
LI18hAM 6.9605 2.8282 1.6328
LI 24hAM 48.3045 13.6289 7.8686
LI 24hHK 2.7208 0.1932 0.1115
L/D 6h 5.5822 0.5218 0.3012
L/D 12h 4.2385 2.544 1.4688
L/D 18h 4.5639 1.8968 1.0951
L/D 24h 3.7565 1.2746 0.7359
Fruiting body development RNA-Seq analysis of fruiting body development Krizsan et al. 2019 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Vegetative mycelium 13.8755 1.6466 0.9507
Hyphal knots 10.2516 2.512 1.4503
Primordium 1 7.2591 1.3895 0.8022
Primordium 2 6.5834 0.6895 0.3981
Young Fruiting body cap 3.1517 0.9117 0.5264
Younf fruiting body gill 3.5843 1.2153 0.7017
Young fruiting body stipe 3.1784 0.3635 0.2099
Mature fruiting body cap_gill 2.9473 1.934 1.1166
Mature fruiting body stipe 2.8234 0.5121 0.2956
Nitrogen sources (12h incubation) Expression profiling on diverse nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Complete medium - control 9.8142 1.002 0.5785
NH4NO3 9.3983 0.4142 0.2391
Proline 7.5318 0.9443 0.5452
Tryptophan 4.2777 1.3244 0.7646
Isoleucin 8.8328 0.6356 0.367
Arginine 10.2915 1.2291 0.7096
Metionine 8.4835 1.6874 0.9742
NaNO3 7.7229 1.2073 0.6971
Plant biomass (12h incubation) Expression profiling on diverse plant biomasses as carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 9.8142 1.002 0.5785
Citrus peel 11.0045 1.9755 1.1406
Poplar leaves 77.4878 14.5837 8.4199
Typha leaves 37.0043 28.092 16.2189
Overwintered Salix leaves 66.0606 27.663 15.9712
Reed flowering 20.8705 2.3405 1.3513
Tigernut 13.2947 1.3745 0.7935
Energy cane 23.5757 5.3178 3.0703
Guar gum 38.0555 16.0035 9.2397
Apple peels 9.6111 2.0287 1.1713
Cellobiose 8.6921 0.6373 0.3679
Corn stalk 16.2178 5.9835 3.4546
Horse manure 25.5798 11.8273 6.8285
Lignin 13.803 5.6046 3.2358
Microcrystalline cellulose 9.4042 3.067 1.7707
Oak leaves 89.004 43.8484 25.3159
Pectin esterified 7.4873 1.5301 0.8834
Poplar sawdust 34.1946 11.3164 6.5335
Wheat bran 15.007 1.7148 0.9901
Chlamydomonas reinhardtii 8.5339 2.2803 1.3165
Vertatryl alcohol 14.4864 1.8033 1.0412
Furfural 8.818 1.941 1.1207
Autoclaved mycelium 10.3024 2.9284 1.6907
Simple and complex carbon and nitrogen sources Expression profiling on diverse complex carbon and nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 11.1709 1.8331 1.0583
Ribose 77.3118 39.6746 22.9061
Mannose 13.8955 3.0125 2.1302
Fructose 20.3449 11.9633 6.907
Arabinose 58.09 61.4771 35.4938
Xylose 17.8404 3.8736 2.2364
Galacturonic acid 44.6687 46.3126 26.7386
Rhamnogalacturonan 14.1263 2.1112 1.2189
Pectin esterified 23.4538 9.8439 5.6834
Polygalacturonic acid 20.9405 1.6615 0.9592
Sodium acetate 116.4727 107.0641 61.8135
No nitrogen 10.6807 0.5356 0.3092
BSA 14.2523 0.466 0.269
Glutamine 17.303 3.2018 1.8486
No phosphate 10.0559 0.6867 0.3964
No carbon 33.2564 3.7825 2.6746
No sulphur 12.3535 1.5394 0.8888
Simple and complex carbon sources (12h incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 9.8142 1.002 0.5785
Amylose 8.1149 0.7249 0.4185
Fucose 10.1315 0.8439 0.4873
Galactose 9.431 1.1606 0.6701
Lactose 12.45 2.6291 1.5179
Maltose 11.548 0.2771 0.16
Mannitol 12.8479 1.7858 1.031
Rhamnose 10.4239 1.0179 0.5877
Sorbitol 9.7596 0.9081 0.5243
Trehalose 11.2001 0.9721 0.5613
Glycerol 11.5741 0.4311 0.2489
Glucuronic acid 10.7854 1.7993 1.0388
Arabinan 11.9528 4.7809 2.7602
Galactan 8.3164 0.9378 0.5415
Galactomannan 7.341 2.2812 1.3171
Glucan 9.6217 1.0891 0.6288
Xylan 9.4097 1.138 0.657
Xyloglucan 8.4802 1.4818 0.8555
Arabinogalactan 11.4099 1.4725 0.8502
Pectin 13.1149 1.7976 1.0379
Rhamnogalacturonan 11.2191 2.3079 1.3324
Polygalacturonic acid 10.5799 1.1238 0.6488
Mannan 8.5371 1.4881 0.8592
Amylopectin 9.9278 1.0882 0.6283
Inulin 10.9676 2.054 1.1859
BSA 15.0647 1.8254 1.0539
Starvation induced by water agar transfer Expression profiling on starvation induced by transfer to water agar Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Control 2.8818 1.3056 0.7538
2h WAT 1.6511 1.0262 0.5925
4h WAT 1.0739 0.4931 0.2847
8h WAT 38.2877 10.907 6.2972
16h WAT 65.5263 5.951 3.4358
24h WAT 38.7023 6.8148 3.9345
WAT hyphal knot -30h 42.6154 22.4176 12.9428
WAT hyphal knot -22h 34.1106 7.2026 4.1584
WAT hyphal knot -15h 27.2684 1.5221 0.8788
WAT hyphal knot -7.5h 16.3342 2.7992 1.6161
WAT hyphal knot 12.9939 7.7654 4.4834
WAT hyphal knot +7.5h 6.5986 2.8625 1.6527
Straw time series Time series expression profiling on straw as a carbon source Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 7.3469 0.9412 0.5434
Straw 3h 7.2526 2.7033 1.5608
Straw 6h 7.1965 1.0653 0.6151
Straw 12h 7.8748 1.5422 0.8904
Straw 24h 16.3441 11.0906 6.4032
Stress conditions 1 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 1.8901 0.2612 0.1508
Frost (-20C, 30min) 2.3304 0.4475 0.2584
Heat shock (47C, 2h) 3.7593 1.0687 0.617
High CO2 2.5744 0.0866 0.05
Drought (4% agar, 12h) 2.2987 0.7756 0.4478
Oxidative (1mM H2O2, 3h) 2.8617 0.9013 0.5204
Acidic (pH4, 3h) 2.6286 0.371 0.2142
Alkaline (pH9, 3h) 1.4697 0.6322 0.365
Osmotic (sorbitol 2h) 2.4157 0.1807 0.1043
CongoRed (3h) 1.4869 0.8964 0.5175
Cobalt chloride control 3.9597 0.4074 0.2352
Cobalt chloride 2mM 3.9062 0.4408 0.2545
Stress conditions 2 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 3.209 1.3474 0.7779
Cold stimulation 3.4764 0.7837 0.4525
Scratched surface 1.6687 0.6158 0.3555
Ca2+, 200mM, 24h 2.9455 0.4534 0.2618
Cu2+, 2mM, 6h 1.8189 0.5934 0.3426
Voriconazole 2.3288 1.1189 0.646
Trichoderma interaction, early 1.4738 0.7805 0.4506
Trichoderma interaction 60h 10.2127 12.0341 6.9479
Cobalt chloride 2mM repeat 1.9391 0.7894 0.4558
Hypoxia 9.3884 6.5556 3.7848
Hypoxia control 23.6265 11.4772 6.6264
Protoplastation control 19.6286 3.5918 2.5398
Protoplastation 3.5h 27.2062 7.5836 5.3624
Protoplastation 1.5h 61.8682 28.8394 20.3925

General data

Systematic name -
Protein id CopciAB_359285.T0
Description Belongs to the glycosyl hydrolase 5 (cellulase A) family

Annotation summary

0 100 200 300 400 443

Conserved domains

Analysis Signature accession Signature description InterPro Accession Start End
Pfam PF00734 Fungal cellulose binding domain IPR000254 25 52
Pfam PF00150 Cellulase (glycosyl hydrolase family 5) IPR001547 114 407

SignalP

Prediction Start End Score
SP(Sec/SPI) 1 21 0.9996

Transmembrane domains

Domain n Start End Length
No records

InterPro

Accession Description
IPR000254 Cellulose-binding domain, fungal
IPR045053 Mannan endo-1,4-beta-mannosidase-like
IPR018087 Glycoside hydrolase, family 5, conserved site
IPR035971 Cellulose-binding domain superfamily
IPR001547 Glycoside hydrolase, family 5
IPR017853 Glycoside hydrolase superfamily

GO

Go id Term Ontology
GO:0005576 extracellular region CC
GO:0005975 carbohydrate metabolic process BP
GO:0030248 cellulose binding MF
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds MF
GO:0071704 organic substance metabolic process BP

KEGG

KEGG Orthology
K19355

EggNOG

COG category Description
G Belongs to the glycosyl hydrolase 5 (cellulase A) family

CAZy

Class Family Subfamily
CBM CBM1
GH GH5 GH5_7

Transcription factor

Group
No records

Conservation of CopciAB_359285 across fungi.

Arrow shows the origin of gene family containing CopciAB_359285.

Protein

Sequence id CopciAB_359285.T0
Sequence >CopciAB_359285.T0
MKLSAGLVSLAIAVTSASAQAVGPWGQCGGSGWSGATTCESGYTCQKHNEWYSQCVPGTSSAPPPPVTPQPSTTA
APPVVTPPPPTSATGFVKTNGTRFVLDGKPYTVVGSNSYWVGLSGHSRDNMNRAFADIAAAGGTTVRTWGFNEVT
AYGGIPYYQIWNGRTPSVNTGANGLQNFDQVIAAAKANGIKLIVALTNNWSDYGGMDVYVRQILNSNNHDLFYTD
PDVKAAFKNYIRAFVGRYVNETGILGWELANEPRCRGSTGTTSGRCTPATITAWAREMSAFIKSIDPNHLVALGD
EGFYNQPGHPVYPYQGGEGIDFDVNLQIDTLDFGTVHAYPEHWGQQGNEVGFGNDWIKDHAESQKRYGKPVILEE
YGVTTNKPAVYTEWLRTIQTSGLAGDLYWQAGSRLPTGSTHDDGFTVYPDQPAYQLLRSHAAAMKARG
Length 443

Coding

Sequence id CopciAB_359285.T0
Sequence >CopciAB_359285.T0
ATGAAGCTCTCTGCTGGACTCGTTTCTTTGGCTATTGCCGTCACCTCTGCCTCTGCTCAAGCTGTAGGCCCTTGG
GGCCAGTGTGGTGGTAGTGGATGGAGTGGTGCAACGACTTGCGAGAGCGGCTATACTTGCCAGAAGCACAACGAA
TGGTACTCTCAATGCGTCCCTGGTACCTCCAGCGCGCCTCCCCCTCCCGTCACTCCCCAGCCGTCGACCACTGCC
GCTCCTCCCGTCGTGACCCCGCCACCTCCCACCTCTGCGACTGGCTTCGTCAAGACCAACGGTACCCGCTTCGTT
TTGGACGGAAAGCCGTACACTGTGGTTGGATCCAACTCGTACTGGGTCGGTCTTTCTGGACACAGCAGGGACAAT
ATGAACCGGGCCTTTGCTGACATTGCAGCTGCTGGAGGCACCACTGTCCGAACCTGGGGTTTCAACGAAGTCACT
GCGTATGGCGGTATCCCCTATTATCAGATTTGGAACGGAAGGACGCCGTCCGTCAACACTGGCGCCAATGGTCTT
CAAAACTTCGACCAGGTCATCGCCGCGGCCAAGGCCAACGGCATCAAGCTCATCGTTGCCCTGACGAACAACTGG
TCCGATTATGGCGGCATGGACGTATATGTGAGACAAATTCTCAACTCCAACAACCACGATTTGTTCTACACGGAC
CCGGACGTCAAGGCGGCCTTCAAGAACTATATTAGGGCTTTCGTCGGGAGATACGTCAATGAAACAGGTATCCTT
GGATGGGAGCTTGCGAACGAACCAAGGTGTCGTGGAAGCACCGGCACCACCTCTGGCAGGTGCACACCGGCCACG
ATTACGGCTTGGGCACGGGAAATGTCTGCCTTCATCAAGTCTATTGACCCCAACCACCTGGTGGCCCTTGGAGAC
GAAGGCTTCTACAACCAACCCGGTCACCCGGTCTACCCATACCAGGGCGGGGAAGGTATTGACTTCGACGTCAAC
CTTCAAATCGATACCCTCGACTTTGGTACTGTCCATGCCTATCCTGAGCACTGGGGTCAGCAAGGCAACGAAGTG
GGCTTCGGAAACGATTGGATCAAGGACCACGCCGAATCCCAGAAGAGATACGGCAAGCCCGTCATCCTTGAGGAA
TACGGCGTAACCACCAACAAGCCAGCCGTTTACACTGAATGGCTCAGGACCATCCAAACCTCCGGCCTTGCTGGG
GACCTCTACTGGCAAGCGGGATCTCGACTCCCAACTGGTAGCACTCATGACGACGGATTCACTGTCTACCCTGAC
CAGCCAGCCTACCAGCTCTTGCGAAGCCACGCTGCCGCCATGAAGGCTCGCGGGTAG
Length 1332

Transcript

Sequence id CopciAB_359285.T0
Sequence >CopciAB_359285.T0
ACCTTTGACTCTCACCTTCAATTTTTCAGTTTCGAAACCAGACAATCTTCCAGGTTTCTAGGACTACTGCGGCCA
CCACCTGAAAAACTGGGCCTTGCGATTGCATCCATATATCTTCTCGTTTTCGATGGAACACAACTTGACGAAGAT
TTTCCGGCGCGTTTTCACAACGAAGCAAAATGATTACACGGGGCTCAGTCTAGGTTTCACGACATTCTCAGACTT
GTGCCCTTGTGACGCCAAAACGGATAGCGACTGGTGCTTCTCATTGCGCCCGTTCCAAAAGCCTTTTAGTGGGAG
CGGTCCGCACCGCGCTTGGCGCTAATCCCTGTTAAAGATGATCCACCAACACCCTTCTTTGGCCTTCTACGCACC
TTCTATAGCATCCATCCGGTTGATCTTCGCGAAATCCCTGTGGGCAACACGCAGGACAATGCATACCGCAGGAAC
TCTGGGCAAATGTGACTTTCGTCGAACGATATAAAGCGGACCGACCGCCATAGGAAGCCGTTAAGGAATTCAGCC
ATCATGAAGCTCTCTGCTGGACTCGTTTCTTTGGCTATTGCCGTCACCTCTGCCTCTGCTCAAGCTGTAGGCCCT
TGGGGCCAGTGTGGTGGTAGTGGATGGAGTGGTGCAACGACTTGCGAGAGCGGCTATACTTGCCAGAAGCACAAC
GAATGGTACTCTCAATGCGTCCCTGGTACCTCCAGCGCGCCTCCCCCTCCCGTCACTCCCCAGCCGTCGACCACT
GCCGCTCCTCCCGTCGTGACCCCGCCACCTCCCACCTCTGCGACTGGCTTCGTCAAGACCAACGGTACCCGCTTC
GTTTTGGACGGAAAGCCGTACACTGTGGTTGGATCCAACTCGTACTGGGTCGGTCTTTCTGGACACAGCAGGGAC
AATATGAACCGGGCCTTTGCTGACATTGCAGCTGCTGGAGGCACCACTGTCCGAACCTGGGGTTTCAACGAAGTC
ACTGCGTATGGCGGTATCCCCTATTATCAGATTTGGAACGGAAGGACGCCGTCCGTCAACACTGGCGCCAATGGT
CTTCAAAACTTCGACCAGGTCATCGCCGCGGCCAAGGCCAACGGCATCAAGCTCATCGTTGCCCTGACGAACAAC
TGGTCCGATTATGGCGGCATGGACGTATATGTGAGACAAATTCTCAACTCCAACAACCACGATTTGTTCTACACG
GACCCGGACGTCAAGGCGGCCTTCAAGAACTATATTAGGGCTTTCGTCGGGAGATACGTCAATGAAACAGGTATC
CTTGGATGGGAGCTTGCGAACGAACCAAGGTGTCGTGGAAGCACCGGCACCACCTCTGGCAGGTGCACACCGGCC
ACGATTACGGCTTGGGCACGGGAAATGTCTGCCTTCATCAAGTCTATTGACCCCAACCACCTGGTGGCCCTTGGA
GACGAAGGCTTCTACAACCAACCCGGTCACCCGGTCTACCCATACCAGGGCGGGGAAGGTATTGACTTCGACGTC
AACCTTCAAATCGATACCCTCGACTTTGGTACTGTCCATGCCTATCCTGAGCACTGGGGTCAGCAAGGCAACGAA
GTGGGCTTCGGAAACGATTGGATCAAGGACCACGCCGAATCCCAGAAGAGATACGGCAAGCCCGTCATCCTTGAG
GAATACGGCGTAACCACCAACAAGCCAGCCGTTTACACTGAATGGCTCAGGACCATCCAAACCTCCGGCCTTGCT
GGGGACCTCTACTGGCAAGCGGGATCTCGACTCCCAACTGGTAGCACTCATGACGACGGATTCACTGTCTACCCT
GACCAGCCAGCCTACCAGCTCTTGCGAAGCCACGCTGCCGCCATGAAGGCTCGCGGGTAGATAGATATACGACTA
TTATGGCGGTCAGTACAAGATAGATCTTTCAAACATAATGTACAAAAAGCCAAGGGCGAATCTCGCCGCTTTACA
ATTCTATGGTGGTGTCCATTTTGATCCATCCTTG
Length 1984

Gene

Sequence id CopciAB_359285.T0
Sequence >CopciAB_359285.T0
ACCTTTGACTCTCACCTTCAATTTTTCAGTTTCGAAACCAGACAATCTTCCAGGTTTCTAGGACTACTGCGGCCA
CCACCTGAAAAACTGGGCCTTGCGATTGCATCCATATATCTTCTCGTTTTCGATGGAACACAACTTGACGAAGAT
TTTCCGGCGCGTTTTCACAACGAAGCAAAATGATTACACGGGGCTCAGTCTAGGTTTCACGACATTCTCAGACTT
GTGCCCTTGTGACGCCAAAACGGATAGCGACTGGTGCTTCTCATTGCGCCCGTTCCAAAAGCCTTTTAGTGGGAG
CGGTCCGCACCGCGCTTGGCGCTAATCCCTGTTAAAGATGATCCACCAACACCCTTCTTTGGCCTTCTACGCACC
TTCTATAGCATCCATCCGGTTGATCTTCGCGAAATCCCTGTGGGCAACACGCAGGACAATGCATACCGCAGGAAC
TCTGGGCAAATGTGACTTTCGTCGAACGATATAAAGCGGACCGACCGCCATAGGAAGCCGTTAAGGAATTCAGCC
ATCATGAAGCTCTCTGCTGGACTCGTTTCTTTGGCTATTGCCGTCACCTCTGCCTCTGCTCAAGCTGTAGGCCCT
TGGGGCCAGTGTGGTGGTAGTGGATGGAGTGGTGCAACGAGTGAGTTCCATTGACTGTTCAGCCAAGAATCACCC
TCACCGTATGGAATGACAGCTTGCGAGAGCGGCTATACTTGCCAGAAGCACAACGAATGGTACTCTCAATGCGTC
CCTGGTACCTCCAGCGCGCCTCCCCCTCCCGTCACTCCCCAGCCGTCGACCACTGCCGCTCCTCCCGTCGTGACC
CCGCCACCTCCCACCTCTGCGACTGGCTTCGTCAAGACCAACGGTACCCGCTTCGTTTTGGACGGAAAGCCGTAC
ACTGTGGTTGGGTGAGCGCCCTAGTCCAAAATCACTTTGAATAGTGCTAACACGTATGCAGATCCAACTCGTACT
GGGTCGGTCTTTCTGGACACAGCAGGGACAATATGAACCGGGCCTTTGCTGACATTGCAGCTGCTGGAGGCACCA
CTGTCCGAACCTGGTGAGTAAGAGGAAATTCAAATAGGTAGTGATTCTGACGAAGGCCGTCTCCAGGGGTTTCAA
CGAAGTCACTGCGTATGGCGGTATCCCCTATTATCAGATTTGGAACGGAAGGACGCCGTCCGTCAACACTGGCGC
CAATGGTCTTCAAAACTTCGGTGAGAGAAGTCCTATGTCGAAAGCGCCTTTGCCATTTGCTGAACCCCTTCTCAG
ACCAGGTCATCGCCGCGGCCAAGGCCAACGGCATCAAGCTCATCGTTGCCCTGTAAGCAAATTCACGAAGAGATG
TCCCAAGGCTACTCAATATTCATACCTACTCGACAGGACGAACAACTGGTCCGATTATGGCGGCATGGACGTATA
TGTGAGACAAATTCTCAACTCCAACAACCACGATTTGTTCTACACGGACCCGGACGTCAAGGTACGTGCCTTCAG
GTTGCCTTTTCTGTGCTAGTTCACGCCACCTGACTCCCGTTTATCTAGGCGGCCTTCAAGAACTATATTAGGGCT
TTCGTCGGGAGATACGTCAATGAAACAGGTATCCTTGGATGGGAGCTTGCGAACGAACCAAGGTGTCGTGGAAGC
ACCGGGTATGAGTCGTTATTTGAAGAACCAAAGTCGGATTTTCTCATCATTTTGTTTAGCACCACCTCTGGCAGG
TGCACACCGGCCACGATTACGGCTTGGGCACGGGAAATGTCTGCCTTCATCAAGTCTATTGACCCCAACCACCTG
GTGGCCCTTGGAGACGAAGGCTTCTACAACCAACCCGGTCACCCGGTCTACCCATACCAGTATGCATGCATTCCT
TGGTAGAGCGTTATGTACAAGACTCATCGGTTCCTTTTTTAGGGGCGGGGAAGGTATTGACTTCGACGTCAACCT
TCAAATCGATACCCTCGACTTTGGTACTGTCCATGTAAGAGCAACTGTTGGTATCGGCGTAGACTCGTACTCACC
ATTTTCACCGAAGGCCTATCCTGAGCACTGGGGTCAGCAAGGCAACGAAGTGGGCTTCGGAAACGATTGGATCAA
GGACCACGCCGAATCCCAGAAGAGATACGGCAAGCCCGTCATCCTTGAGGAATACGGCGTAACCACCAACAAGCC
AGCCGTTTACACTGAATGGCTCAGGACCATCCAAACCTCCGGCCTTGCTGGGGACCTCTACTGGTGGGTAGCATG
TCATGCTCACCGACTCGACGCGACTAACCATTATACAGGCAAGCGGGATCTCGACTCCCAACTGGTAGCACTCAT
GACGACGGATTCACTGTCTACCCTGACCAGCCAGCCTACCAGCTCTTGCGAAGCCACGCTGCCGCCATGAAGGCT
CGCGGGTAGATAGATATACGACTATTATGGCGGTCAGTACAAGATAGATCTTTCAAACATAATGTACAAAAAGCC
AAGGGCGAATCTCGCCGCTTTACAATTCTATGGTGGTGTCCATTTTGATCCATCCTTG
Length 2533