CopciAB_365333
Coprinopsis cinerea A43mutB43mut pab1-1 #326

General data

Systematic name CopciAB_365333 Strain Coprinopsis cinerea Okayama 7
Standard name - Synonyms 365333
Uniprot id Functional description other AgaK1 protein kinase
Location scaffold_7:2058276..2060004 Strand +
Gene length (nt) 1729 Transcript length (nt) 1495
CDS length (nt) 1152 Protein length (aa) 383

Reciprocal best hits in model fungi

Strain name Gene / Protein name

Orthologs in mushroom models

Strain name Gene / Protein name Pident E-value Bits
Ganoderma sp. 10597 SS1 v1.0 Gansp1_65481 39.4 1.361E-83 281
Lentinula edodes NBRC 111202 Lenedo1_1065852 36.6 1.528E-71 246
Lentinula edodes W1-26 v1.0 Lentinedodes1_23193 36.6 1.516E-71 246
Auricularia subglabra Aurde3_1_1240640 33.7 2.931E-54 196
Flammulina velutipes Flave_chr05AA00209 40.9 3.822E-53 192

Expression

Name Summary Attribution Assay type
Carbon metabolism Transcriptional changes along alternative morphogenetic paths of C. cinerea Xie et al. 2020 RNA-Seq
Sample Mean cpm SD cpm Se of mean
sclerotia 68.5176 3.5778 2.0656
oidia 67.4733 5.1096 2.95
vegetative_mycelium 82.091 7.8813 4.5503
primordia 20.7794 2.0161 1.164
hyphal_knot 47.5549 2.5342 1.4631
young_fruiting_body 14.6942 1.116 0.6443
Complex carbon sources 1 (long incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control - glucose 88.6075 8.5863 4.9573
Wheat bran 105.9827 17.8585 10.3106
Horse manure 68.6045 9.3781 5.4144
Oak leaves 79.6251 6.5333 3.772
Hay 87.537 12.1266 7.0013
Corn stalk 80.0774 8.5797 4.9535
Pectine 77.3326 3.9567 2.2844
Microcrystalline cellulose 78.5494 3.0797 1.7781
Xylose 74.2152 6.0584 3.4978
Cellobiose 78.0045 1.7852 1.0307
Lignin 53.3149 9.2104 5.3177
Apple peels 96.1436 8.4115 4.8564
Coprinopsis challenged with bacteria Transcriptional response to Bacillus subtilis and Escherichia coli. Kombrink et al. 2018 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control 108.3153 3.0652 1.7697
Bsubtilis 90.7531 0.9172 0.5295
Ecoli 92.4316 2.2677 1.3092
Early development 1 Time series expression profiling of basidiospore and oidium germination Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
BS 0h 1.1626 0.5169 0.2984
BS 4h 1.1632 0.7065 0.4079
BS 8h 2.0482 0.2674 0.1544
BS 12h 3.8023 0.5894 0.3403
Oidia 0 h 9.7043 2.8453 1.6427
Oidia 18 h 9.0778 0.4535 0.2618
Sclerotia 27.3059 4.1844 2.4159
Early development 2 Time series expression profiling of mycelial growth, light induction and fruiting body initiation. Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
DM 12h 36.356 5.861 3.3838
DM 36h 38.4029 5.0195 2.898
DM 60h 47.7511 3.5293 2.0377
DM 84h 53.885 3.0511 1.7615
DM 108h 54.4965 1.6401 0.9469
DM 132h 49.8408 3.3766 1.9495
DM 156hAE 62.9934 2.3131 1.3355
ELI1hAE 45.2628 5.8627 3.3849
ELI2hAE 50.6842 3.718 2.1466
LI2hAE 52.4289 3.3221 1.918
LI6hAE 56.7363 1.3547 0.7821
LI12hAE 39.6798 5.6442 3.2587
LI18hAE 39.9694 1.1824 0.6827
LI 24hAE 68.9994 5.8579 3.3821
DM 156hAM 52.2255 5.4553 3.1496
ELI1hAM 49.6152 10.8949 6.2902
ELI2hAM 56.6153 5.8376 3.3704
LI2hAM 49.1191 7.6755 4.4315
LI6hAM 48.5925 6.8148 3.9345
LI12hAM 42.4262 9.7868 5.6504
LI18hAM 32.9066 2.9611 1.7096
LI 24hAM 43.161 8.1234 4.69
LI 24hHK 57.3085 11.7395 6.7778
L/D 6h 49.1689 4.8582 2.8049
L/D 12h 52.7516 9.1631 5.2903
L/D 18h 45.2221 5.9507 3.4357
L/D 24h 40.1897 6.3044 3.6398
Fruiting body development RNA-Seq analysis of fruiting body development Krizsan et al. 2019 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Vegetative mycelium 48.8062 9.5696 5.525
Hyphal knots 40.8561 3.5715 2.062
Primordium 1 48.4557 2.8301 1.6339
Primordium 2 42.5928 2.4861 1.4354
Young Fruiting body cap 36.5829 10.4454 6.0306
Younf fruiting body gill 18.9149 2.8062 1.6201
Young fruiting body stipe 54.7894 5.4742 3.1605
Mature fruiting body cap_gill 16.3377 1.0544 0.6087
Mature fruiting body stipe 54.7276 5.0747 2.9299
Nitrogen sources (12h incubation) Expression profiling on diverse nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Complete medium - control 58.7348 2.923 1.6876
NH4NO3 72.3852 5.0899 2.9386
Proline 63.2423 1.3192 0.7617
Tryptophan 77.6108 9.2935 5.3656
Isoleucin 65.1858 5.1096 2.95
Arginine 67.1076 3.695 2.1333
Metionine 68.1984 7.488 4.3232
NaNO3 65.9113 4.3223 2.4955
Plant biomass (12h incubation) Expression profiling on diverse plant biomasses as carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 58.7348 2.923 1.6876
Citrus peel 62.155 7.1984 4.156
Poplar leaves 56.3742 7.5459 4.3566
Typha leaves 62.9334 2.8979 1.6731
Overwintered Salix leaves 55.606 5.721 3.303
Reed flowering 54.4501 10.178 5.8763
Tigernut 62.6388 1.0173 0.5873
Energy cane 57.1977 1.9203 1.1087
Guar gum 69.5794 9.6317 5.5609
Apple peels 65.4022 7.7552 4.4775
Cellobiose 76.4929 5.5472 3.2027
Corn stalk 75.9703 2.9294 1.6913
Horse manure 68.8303 5.2182 3.0127
Lignin 59.15 8.6272 4.9809
Microcrystalline cellulose 64.5419 3.4694 2.0031
Oak leaves 68.4256 2.2872 1.3205
Pectin esterified 69.8773 3.4114 1.9696
Poplar sawdust 76.7147 3.7584 2.1699
Wheat bran 63.797 9.6932 5.5963
Chlamydomonas reinhardtii 61.9083 4.1734 2.4095
Vertatryl alcohol 65.6651 2.7809 1.6055
Furfural 80.7256 6.8242 3.9399
Autoclaved mycelium 58.515 8.1961 4.732
Simple and complex carbon and nitrogen sources Expression profiling on diverse complex carbon and nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 93.2371 7.2831 4.2049
Ribose 92.2049 3.3394 1.928
Mannose 92.928 2.9436 2.0814
Fructose 91.6606 7.4992 4.3297
Arabinose 93.2594 8.2802 4.7806
Xylose 84.5354 9.4315 5.4453
Galacturonic acid 90.3901 4.0447 2.3352
Rhamnogalacturonan 86.2535 1.4656 0.8462
Pectin esterified 88.9221 3.267 1.8862
Polygalacturonic acid 91.0746 2.0529 1.1853
Sodium acetate 106.7672 10.352 5.9767
No nitrogen 89.1055 1.1724 0.6769
BSA 89.9673 5.5153 3.1842
Glutamine 84.6089 5.9792 3.4521
No phosphate 84.1461 3.3824 1.9529
No carbon 100.3631 8.6452 6.1131
No sulphur 91.3896 15.7401 9.0876
Simple and complex carbon sources (12h incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 58.7348 2.923 1.6876
Amylose 66.3302 6.4507 3.7243
Fucose 65.3793 6.3422 3.6617
Galactose 70.2276 3.302 1.9064
Lactose 60.6601 4.686 2.7055
Maltose 60.0225 2.4931 1.4394
Mannitol 58.4726 0.5077 0.2931
Rhamnose 60.634 3.7723 2.1779
Sorbitol 68.9414 3.742 2.1605
Trehalose 60.5593 3.4457 1.9894
Glycerol 63.5018 5.4392 3.1403
Glucuronic acid 73.8534 4.3014 2.4834
Arabinan 69.3183 4.8208 2.7833
Galactan 64.946 4.236 2.4457
Galactomannan 75.6033 16.3463 9.4375
Glucan 70.102 13.7453 7.9359
Xylan 75.3711 3.6115 2.0851
Xyloglucan 74.2462 3.3587 1.9392
Arabinogalactan 70.7265 5.3699 3.1003
Pectin 62.8312 5.7492 3.3193
Rhamnogalacturonan 40.6839 5.7797 3.3369
Polygalacturonic acid 67.294 1.8944 1.0937
Mannan 69.3645 5.9202 3.418
Amylopectin 61.6406 5.6308 3.251
Inulin 62.2452 2.4077 1.3901
BSA 42.4367 0.5793 0.3344
Starvation induced by water agar transfer Expression profiling on starvation induced by transfer to water agar Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Control 47.7511 3.5293 2.0377
2h WAT 45.3459 2.3354 1.3483
4h WAT 45.5246 3.3568 1.938
8h WAT 40.3377 2.6741 1.5439
16h WAT 40.8546 5.696 3.2886
24h WAT 37.6072 3.5194 2.0319
WAT hyphal knot -30h 40.9067 4.9778 2.874
WAT hyphal knot -22h 35.9958 2.4278 1.4017
WAT hyphal knot -15h 31.7594 3.6488 2.1067
WAT hyphal knot -7.5h 34.0281 8.4992 4.907
WAT hyphal knot 38.6872 2.3534 1.3587
WAT hyphal knot +7.5h 37.2747 4.7235 2.7271
Straw time series Time series expression profiling on straw as a carbon source Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 60.8486 10.0846 5.8224
Straw 3h 62.914 6.6967 3.8663
Straw 6h 61.0485 9.9627 5.752
Straw 12h 63.6812 3.7668 2.1748
Straw 24h 64.1045 0.4239 0.2448
Stress conditions 1 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 37.8067 6.8241 3.9399
Frost (-20C, 30min) 50.126 3.8839 2.2424
Heat shock (47C, 2h) 18.5119 3.3096 1.9108
High CO2 52.0312 2.7755 1.6024
Drought (4% agar, 12h) 40.5 5.3316 3.0782
Oxidative (1mM H2O2, 3h) 31.7184 3.8715 2.2352
Acidic (pH4, 3h) 39.091 2.7073 1.5631
Alkaline (pH9, 3h) 46.8193 3.7852 2.1854
Osmotic (sorbitol 2h) 30.8277 4.0658 2.3474
CongoRed (3h) 40.1417 3.9393 2.2743
Cobalt chloride control 40.7914 1.4915 0.8611
Cobalt chloride 2mM 36.0061 4.4614 2.5758
Stress conditions 2 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 53.7544 12.5428 7.2416
Cold stimulation 45.0957 3.5127 2.0281
Scratched surface 52.4473 5.4006 3.1181
Ca2+, 200mM, 24h 33.9807 4.9032 2.8308
Cu2+, 2mM, 6h 29.7252 0.8825 0.5095
Voriconazole 30.9005 6.4125 3.7023
Trichoderma interaction, early 40.8012 0.8833 0.51
Trichoderma interaction 60h 14.4848 13.3252 7.6933
Cobalt chloride 2mM repeat 31.185 1.5013 0.8668
Hypoxia 45.1596 7.6784 4.4331
Hypoxia control 41.6082 2.66 1.5358
Protoplastation control 36.6628 3.4397 2.4322
Protoplastation 3.5h 32.008 6.0018 4.2439
Protoplastation 1.5h 36.5812 18.2562 12.9091

General data

Systematic name -
Protein id CopciAB_365333.T0
Description other AgaK1 protein kinase

Annotation summary

Conserved domains

Analysis Signature accession Signature description InterPro Accession Start End
No records

SignalP

Prediction Start End Score
No records

Transmembrane domains

Domain n Start End Length
No records

InterPro

Accession Description
IPR011009 Protein kinase-like domain superfamily
IPR000719 Protein kinase domain

GO

Go id Term Ontology
GO:0004672 protein kinase activity MF
GO:0005524 ATP binding MF
GO:0006468 protein phosphorylation BP

KEGG

KEGG Orthology
No records

EggNOG

COG category Description
G other AgaK1 protein kinase

CAZy

Class Family Subfamily
No records

Transcription factor

Group
No records

Conservation of CopciAB_365333 across fungi.

Arrow shows the origin of gene family containing CopciAB_365333.

Protein

Sequence id CopciAB_365333.T0
Sequence >CopciAB_365333.T0
MGVFDELGRSEVYWRDHYEWFKEQGFELRARYKPGWIPSWKTDPTLKPGLSEDSEMFNRSHLNDATRIADGKHVV
LKRIEKSQYPEEVSILRYFSEKPLASNPENHCVPVLAVLNPPDDPEHDIAVLPILRDYDDPPFETIGEVVDYIGQ
ILEGFVFLHEHRVAHRDVRRDNIMMDPHAMGHSSWHFVCPEKTRDYRSEVTHKYTRTERRPRYYIIDFGYSAQFK
PEEMPPSTLPKPASDTSLPEYNNLWKPCDPFPIDVYVVGNMIRMDFLDGYPKEGFLSYRHCEKMPSTKRLEFLRP
LIDAMIKENPNERVTMKEAFSLFNDIVSKVGNSTLRRRPDVLGGLPRHEWFYERIANTASHWYKTMRCVICRIPP
IPRERTAP
Length 383

Coding

Sequence id CopciAB_365333.T0
Sequence >CopciAB_365333.T0
ATGGGTGTTTTCGACGAACTTGGTCGATCCGAGGTCTATTGGAGAGATCATTATGAATGGTTCAAGGAGCAGGGG
TTTGAACTTCGAGCCCGCTATAAACCAGGGTGGATTCCCTCTTGGAAAACAGACCCCACCTTGAAGCCGGGGCTC
AGCGAGGATTCGGAAATGTTCAATCGCAGCCACCTCAACGATGCTACCCGAATCGCCGACGGCAAGCACGTCGTT
TTAAAACGAATCGAAAAGTCTCAATATCCAGAAGAAGTGTCTATCCTCAGGTATTTCTCAGAAAAACCACTTGCT
TCCAACCCAGAGAACCATTGTGTTCCTGTTCTGGCGGTCTTGAACCCTCCTGACGACCCCGAGCACGACATTGCT
GTCCTACCGATCTTACGGGATTACGACGACCCTCCTTTCGAAACCATTGGTGAGGTGGTCGACTACATCGGGCAG
ATTCTAGAGGGATTCGTGTTCCTCCACGAACATCGTGTTGCACATCGAGATGTCCGAAGGGACAACATCATGATG
GACCCTCATGCCATGGGCCACAGTTCATGGCATTTTGTCTGCCCCGAAAAGACGCGCGACTACAGGTCAGAAGTT
ACTCACAAGTACACGCGCACCGAGCGTAGGCCAAGGTATTATATCATTGACTTTGGGTACTCTGCCCAGTTCAAA
CCCGAGGAGATGCCGCCGTCGACTCTGCCAAAACCGGCCAGCGACACCAGCCTTCCTGAATATAACAATCTATGG
AAGCCGTGCGATCCATTTCCGATCGATGTGTATGTCGTAGGAAATATGATTCGTATGGACTTTTTGGATGGCTAC
CCGAAGGAAGGTTTCCTCAGCTATCGCCATTGCGAGAAGATGCCCAGCACAAAACGCCTTGAGTTTCTTCGACCG
TTAATAGACGCGATGATTAAGGAGAACCCGAACGAAAGGGTCACGATGAAGGAGGCATTCTCTCTCTTTAACGAC
ATCGTGTCAAAGGTTGGCAACAGTACGCTACGGCGTAGGCCTGACGTGCTTGGTGGGCTCCCTCGTCATGAGTGG
TTTTACGAGCGCATCGCCAATACAGCCTCGCACTGGTACAAGACGATGCGTTGTGTCATTTGTAGGATTCCTCCC
ATTCCGAGAGAACGCACGGCACCGTGA
Length 1152

Transcript

Sequence id CopciAB_365333.T0
Sequence >CopciAB_365333.T0
ATCTCACTTTGAACACACTGTTGACGGACACCCGAGTCATGGGTGTTTTCGACGAACTTGGTCGATCCGAGGTCT
ATTGGAGAGATCATTATGAATGGTTCAAGGAGCAGGGGTTTGAACTTCGAGCCCGCTATAAACCAGGGTGGATTC
CCTCTTGGAAAACAGACCCCACCTTGAAGCCGGGGCTCAGCGAGGATTCGGAAATGTTCAATCGCAGCCACCTCA
ACGATGCTACCCGAATCGCCGACGGCAAGCACGTCGTTTTAAAACGAATCGAAAAGTCTCAATATCCAGAAGAAG
TGTCTATCCTCAGGTATTTCTCAGAAAAACCACTTGCTTCCAACCCAGAGAACCATTGTGTTCCTGTTCTGGCGG
TCTTGAACCCTCCTGACGACCCCGAGCACGACATTGCTGTCCTACCGATCTTACGGGATTACGACGACCCTCCTT
TCGAAACCATTGGTGAGGTGGTCGACTACATCGGGCAGATTCTAGAGGGATTCGTGTTCCTCCACGAACATCGTG
TTGCACATCGAGATGTCCGAAGGGACAACATCATGATGGACCCTCATGCCATGGGCCACAGTTCATGGCATTTTG
TCTGCCCCGAAAAGACGCGCGACTACAGGTCAGAAGTTACTCACAAGTACACGCGCACCGAGCGTAGGCCAAGGT
ATTATATCATTGACTTTGGGTACTCTGCCCAGTTCAAACCCGAGGAGATGCCGCCGTCGACTCTGCCAAAACCGG
CCAGCGACACCAGCCTTCCTGAATATAACAATCTATGGAAGCCGTGCGATCCATTTCCGATCGATGTGTATGTCG
TAGGAAATATGATTCGTATGGACTTTTTGGATGGCTACCCGAAGGAAGGTTTCCTCAGCTATCGCCATTGCGAGA
AGATGCCCAGCACAAAACGCCTTGAGTTTCTTCGACCGTTAATAGACGCGATGATTAAGGAGAACCCGAACGAAA
GGGTCACGATGAAGGAGGCATTCTCTCTCTTTAACGACATCGTGTCAAAGGTTGGCAACAGTACGCTACGGCGTA
GGCCTGACGTGCTTGGTGGGCTCCCTCGTCATGAGTGGTTTTACGAGCGCATCGCCAATACAGCCTCGCACTGGT
ACAAGACGATGCGTTGTGTCATTTGTAGGATTCCTCCCATTCCGAGAGAACGCACGGCACCGTGACTGGACACAT
CTTATGCACATATATCAGCACCTGCACCTACAGTCTATGTTTAGTTTCAAATGTTTCTAGGTATCCATCGGGTGT
ACATTCTCAGTCCTGCTGCGATGTACTCCTGTTTGAACCACCCGCTCGTGCCAAGGTTATGTAGCCATTGTCCGG
CCATATCGCTCGTACTGTTATTCACCCATAACGTTTCCAGTCCGTTTGTTCGACTACCTATGATATATGATCTAT
CGTACACGGTGTTCTCGCTCATGAACTAGTGGCCTTTTTATCTCGTAATTCAACACTCCCATTGCACCTC
Length 1495

Gene

Sequence id CopciAB_365333.T0
Sequence >CopciAB_365333.T0
ATCTCACTTTGAACACACTGTTGACGGACACCCGAGTCATGGGTGTTTTCGACGAACTTGGTCGATCCGAGGTCT
ATTGGAGAGATCATTATGAATGGTTCAAGGAGCAGGGGTTTGAACTTCGAGCCCGCTATAAACCAGGGTGGATTC
CCTCTTGGAAAACAGACCCCACCTTGAAGCCGGGGCTCAGCGAGGATTCGGAAATGTTCAATGTACGTTCCCTGC
TCTTTCATTTCAGTGTAGTTTACGGCTAAAACGTTTCACTTCAAGCGCAGCCACCTCAACGATGCTACCCGAATC
GCCGACGGCAAGCACGTCGTTTTAAAACGAATCGAAAAGTCTCAATATCCAGAAGAAGTGTCTATCCTCAGGTAT
TTCTCAGAAAAACCACTTGCTTCCAACCCAGAGAACCATTGTGTTCCTGTTCTGGCGGTCTTGAACCCTCCTGAC
GACCCCGAGCACGACATTGCTGTCCTACCGATCTTACGGGATTACGACGACCCTCCTTTCGAAACCATTGGTGAG
GTGGTCGACTACATCGGGCAGATTCTAGAGGTACACAGACGCAGACACAATGATTAGACACCCTTGTCCTGACCT
CCGTTTTCTCCAGGGATTCGTGTTCCTCCACGAACATCGTGTTGCACATCGGTCCGTGTCAGTACTCCTTGCGAG
AAAGCGCCCACTAACTCCTCCAATATTCAGAGATGTCCGAAGGGACAACATCATGATGGACCCTCATGCCATGGG
CCACAGTTCATGGCATTTTGTCTGCCCCGAAAAGACGCGCGACTACAGGTCAGAAGTTACTCACAAGTACACGCG
CACCGAGCGTAGGCCAAGGTATTATATCATTGACTTTGGGTACTCTGCCCAGTTCAAACCCGAGGAGATGCCGCC
GTCGACTCTGCCAAAACCGGCCAGCGACACCAGCCTTCCTGAATATAACAATCTATGGAAGCCGTGCGATCCATT
TCCGATCGATGTGTATGTCGTAGGAAATATGATTCGTATGGACTTTTTGGATGTAGGGGTTGCTTTCTTTCCTTA
ATCATTGTTTCTCAAATCCCTGATCCATCCCCGTTCACCAGGGCTACCCGAAGGAAGGTTTCCTCAGCTATCGCC
ATTGCGAGAAGATGCCCAGCACAAAACGCCTTGAGTTTCTTCGACCGTTAATAGACGCGATGATTAAGGAGAACC
CGAACGAAAGGGTCACGATGAAGGAGGCATTCTCTCTCTTTAACGACATCGTGTCAAAGGTTGGCAACAGTACGC
TACGGCGTAGGCCTGACGTGCTTGGTGGGCTCCCTCGTCATGAGTGGTTTTACGAGCGCATCGCCAATACAGCCT
CGCACTGGTACAAGACGATGCGTTGTGTCATTTGTAGGATTCCTCCCATTCCGAGAGAACGCACGGCACCGTGAC
TGGACACATCTTATGCACATATATCAGCACCTGCACCTACAGTCTATGTTTAGTTTCAAATGTTTCTAGGTATCC
ATCGGGTGTACATTCTCAGTCCTGCTGCGATGTACTCCTGTTTGAACCACCCGCTCGTGCCAAGGTTATGTAGCC
ATTGTCCGGCCATATCGCTCGTACTGTTATTCACCCATAACGTTTCCAGTCCGTTTGTTCGACTACCTATGATAT
ATGATCTATCGTACACGGTGTTCTCGCTCATGAACTAGTGGCCTTTTTATCTCGTAATTCAACACTCCCATTGCA
CCTC
Length 1729