|
Carbon metabolism |
Transcriptional changes along alternative morphogenetic paths of C. cinerea |
Xie et al. 2020
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
sclerotia |
0 |
0 |
0 |
oidia |
0.1578 |
0.0426 |
0.0246 |
vegetative_mycelium |
0.1352 |
0.1823 |
0.1052 |
primordia |
0.1653 |
0.2257 |
0.1303 |
hyphal_knot |
0.0456 |
0.079 |
0.0456 |
young_fruiting_body |
0.0859 |
0.1487 |
0.0859 |
|
|
Complex carbon sources 1 (long incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control - glucose |
0.1745 |
0.0215 |
0.0124 |
Wheat bran |
0.2023 |
0.1492 |
0.0861 |
Horse manure |
0.1042 |
0.0918 |
0.053 |
Oak leaves |
0 |
0 |
0 |
Hay |
0.05 |
0.0866 |
0.05 |
Corn stalk |
0.0623 |
0.0587 |
0.0339 |
Pectine |
0.1364 |
0.0535 |
0.0309 |
Microcrystalline cellulose |
0.0907 |
0.1572 |
0.0907 |
Xylose |
0.0303 |
0.0525 |
0.0303 |
Cellobiose |
0.3544 |
0.0575 |
0.0332 |
Lignin |
0.05 |
0.0457 |
0.0264 |
Apple peels |
0.0918 |
0.0855 |
0.0494 |
|
|
Coprinopsis challenged with bacteria |
Transcriptional response to Bacillus subtilis and Escherichia coli. |
Kombrink et al. 2018
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
0.1102 |
0.0282 |
0.0163 |
Bsubtilis |
0.0349 |
0.0303 |
0.0175 |
Ecoli |
0.189 |
0.0805 |
0.0465 |
|
|
Early development 1 |
Time series expression profiling of basidiospore and oidium germination |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
BS 0h |
0.1097 |
0.0955 |
0.0552 |
BS 4h |
0.0209 |
0.0362 |
0.0209 |
BS 8h |
0 |
0 |
0 |
BS 12h |
0 |
0 |
0 |
Oidia 0 h |
0 |
0 |
0 |
Oidia 18 h |
0 |
0 |
0 |
Sclerotia |
0 |
0 |
0 |
|
|
Early development 2 |
Time series expression profiling of mycelial growth, light induction and fruiting body initiation. |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
DM 12h |
0.0264 |
0.0457 |
0.0264 |
DM 36h |
0 |
0 |
0 |
DM 60h |
0 |
0 |
0 |
DM 84h |
0 |
0 |
0 |
DM 108h |
0 |
0 |
0 |
DM 132h |
0 |
0 |
0 |
DM 156hAE |
0 |
0 |
0 |
ELI1hAE |
0 |
0 |
0 |
ELI2hAE |
0 |
0 |
0 |
LI2hAE |
0 |
0 |
0 |
LI6hAE |
0 |
0 |
0 |
LI12hAE |
0 |
0 |
0 |
LI18hAE |
0.0581 |
0.0685 |
0.0395 |
LI 24hAE |
0.0815 |
0.1411 |
0.0815 |
DM 156hAM |
0.2728 |
0.4724 |
0.2728 |
ELI1hAM |
0 |
0 |
0 |
ELI2hAM |
0.0046 |
0.008 |
0.0046 |
LI2hAM |
0 |
0 |
0 |
LI6hAM |
0 |
0 |
0 |
LI12hAM |
0 |
0 |
0 |
LI18hAM |
0.0203 |
0.0352 |
0.0203 |
LI 24hAM |
0 |
0 |
0 |
LI 24hHK |
0 |
0 |
0 |
L/D 6h |
0.1604 |
0.2778 |
0.1604 |
L/D 12h |
0.1595 |
0.2098 |
0.1211 |
L/D 18h |
0 |
0 |
0 |
L/D 24h |
0 |
0 |
0 |
|
|
Fruiting body development |
RNA-Seq analysis of fruiting body development |
Krizsan et al. 2019
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Vegetative mycelium |
0.0842 |
0.0772 |
0.0446 |
Hyphal knots |
0.1038 |
0.0904 |
0.0522 |
Primordium 1 |
0.1119 |
0.071 |
0.041 |
Primordium 2 |
0.1693 |
0.0798 |
0.0461 |
Young Fruiting body cap |
0.0521 |
0.0512 |
0.0295 |
Younf fruiting body gill |
0.0538 |
0.0466 |
0.0269 |
Young fruiting body stipe |
0.4484 |
0.2061 |
0.119 |
Mature fruiting body cap_gill |
0.8693 |
0.8445 |
0.4876 |
Mature fruiting body stipe |
0.4377 |
0.2697 |
0.1557 |
|
|
Nitrogen sources (12h incubation) |
Expression profiling on diverse nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Complete medium - control |
0.0193 |
0.0334 |
0.0193 |
NH4NO3 |
0.1271 |
0.1829 |
0.1056 |
Proline |
0.0818 |
0.0277 |
0.016 |
Tryptophan |
0.0854 |
0.1479 |
0.0854 |
Isoleucin |
0.0634 |
0.0771 |
0.0445 |
Arginine |
0.1611 |
0.1966 |
0.1135 |
Metionine |
0.0919 |
0.067 |
0.0387 |
NaNO3 |
0.0216 |
0.0374 |
0.0216 |
|
|
Plant biomass (12h incubation) |
Expression profiling on diverse plant biomasses as carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
0.0193 |
0.0334 |
0.0193 |
Citrus peel |
0.0558 |
0.0966 |
0.0558 |
Poplar leaves |
0.0373 |
0.0323 |
0.0186 |
Typha leaves |
0.0578 |
0.0615 |
0.0355 |
Overwintered Salix leaves |
0.0354 |
0.0308 |
0.0178 |
Reed flowering |
0.0802 |
0.0938 |
0.0542 |
Tigernut |
0.0184 |
0.0318 |
0.0184 |
Energy cane |
0.0413 |
0.0366 |
0.0211 |
Guar gum |
0.0775 |
0.0261 |
0.0151 |
Apple peels |
0.1143 |
0.0476 |
0.0275 |
Cellobiose |
0.1486 |
0.1287 |
0.0743 |
Corn stalk |
0.1204 |
0.1718 |
0.0992 |
Horse manure |
0.4051 |
0.5861 |
0.3384 |
Lignin |
0.0561 |
0.0972 |
0.0561 |
Microcrystalline cellulose |
0 |
0 |
0 |
Oak leaves |
0.2007 |
0.1781 |
0.1028 |
Pectin esterified |
0.1149 |
0.0872 |
0.0503 |
Poplar sawdust |
0.0259 |
0.0448 |
0.0259 |
Wheat bran |
0.1066 |
0.1053 |
0.0608 |
Chlamydomonas reinhardtii |
0.0853 |
0.1018 |
0.0588 |
Vertatryl alcohol |
0.1361 |
0.0872 |
0.0504 |
Furfural |
0.0197 |
0.0341 |
0.0197 |
Autoclaved mycelium |
0.09 |
0.0837 |
0.0483 |
|
|
Simple and complex carbon and nitrogen sources |
Expression profiling on diverse complex carbon and nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
0.2557 |
0.0749 |
0.0432 |
Ribose |
0 |
0 |
0 |
Mannose |
0.4365 |
0.354 |
0.2503 |
Fructose |
0.114 |
0.1543 |
0.0891 |
Arabinose |
0.1527 |
0.0653 |
0.0377 |
Xylose |
0.0918 |
0.0977 |
0.0564 |
Galacturonic acid |
0.1148 |
0.1988 |
0.1148 |
Rhamnogalacturonan |
0.2305 |
0.1642 |
0.0948 |
Pectin esterified |
0.1753 |
0.1003 |
0.0579 |
Polygalacturonic acid |
0.3532 |
0.2143 |
0.1237 |
Sodium acetate |
0.1923 |
0.1286 |
0.0742 |
No nitrogen |
0.1027 |
0.1032 |
0.0596 |
BSA |
0.2141 |
0.1043 |
0.0602 |
Glutamine |
0.1499 |
0.0758 |
0.0438 |
No phosphate |
0.2152 |
0.2308 |
0.1333 |
No carbon |
0.1523 |
0.0506 |
0.0358 |
No sulphur |
0.2058 |
0.1183 |
0.0683 |
|
|
Simple and complex carbon sources (12h incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
0.0193 |
0.0334 |
0.0193 |
Amylose |
0.0306 |
0.053 |
0.0306 |
Fucose |
0.0178 |
0.0308 |
0.0178 |
Galactose |
0.0698 |
0.1208 |
0.0698 |
Lactose |
0.1128 |
0.0346 |
0.02 |
Maltose |
0.0498 |
0.0471 |
0.0272 |
Mannitol |
0.0644 |
0.0709 |
0.0409 |
Rhamnose |
0.0794 |
0.0277 |
0.016 |
Sorbitol |
0.0415 |
0.0431 |
0.0249 |
Trehalose |
0.132 |
0.127 |
0.0733 |
Glycerol |
0.0973 |
0.0157 |
0.009 |
Glucuronic acid |
0.0704 |
0.0632 |
0.0365 |
Arabinan |
0.0451 |
0.0457 |
0.0264 |
Galactan |
0.0553 |
0.0014 |
0.0008 |
Galactomannan |
0.039 |
0.0675 |
0.039 |
Glucan |
0.081 |
0.0702 |
0.0405 |
Xylan |
0.0626 |
0.026 |
0.015 |
Xyloglucan |
0.1838 |
0.1501 |
0.0867 |
Arabinogalactan |
0.0512 |
0.0886 |
0.0512 |
Pectin |
0.0699 |
0.0686 |
0.0396 |
Rhamnogalacturonan |
0.03 |
0.052 |
0.03 |
Polygalacturonic acid |
0.0344 |
0.0596 |
0.0344 |
Mannan |
0.1557 |
0.1106 |
0.0638 |
Amylopectin |
0.0445 |
0.0397 |
0.0229 |
Inulin |
0.1133 |
0.067 |
0.0387 |
BSA |
0.1398 |
0.1267 |
0.0731 |
|
|
Starvation induced by water agar transfer |
Expression profiling on starvation induced by transfer to water agar |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
0 |
0 |
0 |
2h WAT |
0.0335 |
0.0581 |
0.0335 |
4h WAT |
0 |
0 |
0 |
8h WAT |
0 |
0 |
0 |
16h WAT |
0 |
0 |
0 |
24h WAT |
0.011 |
0.019 |
0.011 |
WAT hyphal knot -30h |
0 |
0 |
0 |
WAT hyphal knot -22h |
0 |
0 |
0 |
WAT hyphal knot -15h |
0 |
0 |
0 |
WAT hyphal knot -7.5h |
0 |
0 |
0 |
WAT hyphal knot |
0 |
0 |
0 |
WAT hyphal knot +7.5h |
0 |
0 |
0 |
|
|
Straw time series |
Time series expression profiling on straw as a carbon source |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
0.0874 |
0.0837 |
0.0483 |
Straw 3h |
0.0158 |
0.0274 |
0.0158 |
Straw 6h |
0.1407 |
0.0879 |
0.0507 |
Straw 12h |
0.0156 |
0.027 |
0.0156 |
Straw 24h |
0.0363 |
0.0329 |
0.019 |
|
|
Stress conditions 1 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
0 |
0 |
0 |
Frost (-20C, 30min) |
0.0041 |
0.0071 |
0.0041 |
Heat shock (47C, 2h) |
0 |
0 |
0 |
High CO2 |
0 |
0 |
0 |
Drought (4% agar, 12h) |
0 |
0 |
0 |
Oxidative (1mM H2O2, 3h) |
0 |
0 |
0 |
Acidic (pH4, 3h) |
0.0113 |
0.0195 |
0.0113 |
Alkaline (pH9, 3h) |
0 |
0 |
0 |
Osmotic (sorbitol 2h) |
0 |
0 |
0 |
CongoRed (3h) |
0.0438 |
0.0759 |
0.0438 |
Cobalt chloride control |
0 |
0 |
0 |
Cobalt chloride 2mM |
0.0201 |
0.0347 |
0.0201 |
|
|
Stress conditions 2 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
0.0322 |
0.0558 |
0.0322 |
Cold stimulation |
0.0077 |
0.0134 |
0.0077 |
Scratched surface |
0 |
0 |
0 |
Ca2+, 200mM, 24h |
0.3587 |
0.6213 |
0.3587 |
Cu2+, 2mM, 6h |
0.1188 |
0.2057 |
0.1188 |
Voriconazole |
0.1687 |
0.1757 |
0.1015 |
Trichoderma interaction, early |
0 |
0 |
0 |
Trichoderma interaction 60h |
0 |
0 |
0 |
Cobalt chloride 2mM repeat |
0 |
0 |
0 |
Hypoxia |
0 |
0 |
0 |
Hypoxia control |
0 |
0 |
0 |
Protoplastation control |
0 |
0 |
0 |
Protoplastation 3.5h |
0 |
0 |
0 |
Protoplastation 1.5h |
0 |
0 |
0 |
|