CopciAB_371582
Coprinopsis cinerea A43mutB43mut pab1-1 #326

General data

Systematic name CopciAB_371582 Strain Coprinopsis cinerea Okayama 7
Standard name - Synonyms 371582
Uniprot id Functional description Atypical alpha protein kinase
Location scaffold_4:542476..543266 Strand +
Gene length (nt) 791 Transcript length (nt) 594
CDS length (nt) 594 Protein length (aa) 197

Reciprocal best hits in model fungi

Strain name Gene / Protein name

Orthologs in mushroom models

Strain name Gene / Protein name Pident E-value Bits
Pleurotus ostreatus PC9 PleosPC9_1_111631 37.3 4.403E-37 137
Pleurotus eryngii ATCC 90797 Pleery1_1509315 34 1.49E-33 127
Hypsizygus marmoreus strain 51987-8 Hypma_RDB26567 33.6 3.805E-22 94
Auricularia subglabra Aurde3_1_1315160 37.9 5.88E-20 88
Lentinula edodes B17 Lened_B_1_1_12336 32.3 3.337E-17 79
Schizophyllum commune H4-8 Schco3_1083424 33.3 4.99E-16 76
Lentinula edodes W1-26 v1.0 Lentinedodes1_7235 45.5 9.866E-15 72

Expression

Name Summary Attribution Assay type
Carbon metabolism Transcriptional changes along alternative morphogenetic paths of C. cinerea Xie et al. 2020 RNA-Seq
Sample Mean cpm SD cpm Se of mean
sclerotia 0 0 0
oidia 0.1578 0.0426 0.0246
vegetative_mycelium 0.1352 0.1823 0.1052
primordia 0.1653 0.2257 0.1303
hyphal_knot 0.0456 0.079 0.0456
young_fruiting_body 0.0859 0.1487 0.0859
Complex carbon sources 1 (long incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control - glucose 0.1745 0.0215 0.0124
Wheat bran 0.2023 0.1492 0.0861
Horse manure 0.1042 0.0918 0.053
Oak leaves 0 0 0
Hay 0.05 0.0866 0.05
Corn stalk 0.0623 0.0587 0.0339
Pectine 0.1364 0.0535 0.0309
Microcrystalline cellulose 0.0907 0.1572 0.0907
Xylose 0.0303 0.0525 0.0303
Cellobiose 0.3544 0.0575 0.0332
Lignin 0.05 0.0457 0.0264
Apple peels 0.0918 0.0855 0.0494
Coprinopsis challenged with bacteria Transcriptional response to Bacillus subtilis and Escherichia coli. Kombrink et al. 2018 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control 0.1102 0.0282 0.0163
Bsubtilis 0.0349 0.0303 0.0175
Ecoli 0.189 0.0805 0.0465
Early development 1 Time series expression profiling of basidiospore and oidium germination Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
BS 0h 0.1097 0.0955 0.0552
BS 4h 0.0209 0.0362 0.0209
BS 8h 0 0 0
BS 12h 0 0 0
Oidia 0 h 0 0 0
Oidia 18 h 0 0 0
Sclerotia 0 0 0
Early development 2 Time series expression profiling of mycelial growth, light induction and fruiting body initiation. Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
DM 12h 0.0264 0.0457 0.0264
DM 36h 0 0 0
DM 60h 0 0 0
DM 84h 0 0 0
DM 108h 0 0 0
DM 132h 0 0 0
DM 156hAE 0 0 0
ELI1hAE 0 0 0
ELI2hAE 0 0 0
LI2hAE 0 0 0
LI6hAE 0 0 0
LI12hAE 0 0 0
LI18hAE 0.0581 0.0685 0.0395
LI 24hAE 0.0815 0.1411 0.0815
DM 156hAM 0.2728 0.4724 0.2728
ELI1hAM 0 0 0
ELI2hAM 0.0046 0.008 0.0046
LI2hAM 0 0 0
LI6hAM 0 0 0
LI12hAM 0 0 0
LI18hAM 0.0203 0.0352 0.0203
LI 24hAM 0 0 0
LI 24hHK 0 0 0
L/D 6h 0.1604 0.2778 0.1604
L/D 12h 0.1595 0.2098 0.1211
L/D 18h 0 0 0
L/D 24h 0 0 0
Fruiting body development RNA-Seq analysis of fruiting body development Krizsan et al. 2019 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Vegetative mycelium 0.0842 0.0772 0.0446
Hyphal knots 0.1038 0.0904 0.0522
Primordium 1 0.1119 0.071 0.041
Primordium 2 0.1693 0.0798 0.0461
Young Fruiting body cap 0.0521 0.0512 0.0295
Younf fruiting body gill 0.0538 0.0466 0.0269
Young fruiting body stipe 0.4484 0.2061 0.119
Mature fruiting body cap_gill 0.8693 0.8445 0.4876
Mature fruiting body stipe 0.4377 0.2697 0.1557
Nitrogen sources (12h incubation) Expression profiling on diverse nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Complete medium - control 0.0193 0.0334 0.0193
NH4NO3 0.1271 0.1829 0.1056
Proline 0.0818 0.0277 0.016
Tryptophan 0.0854 0.1479 0.0854
Isoleucin 0.0634 0.0771 0.0445
Arginine 0.1611 0.1966 0.1135
Metionine 0.0919 0.067 0.0387
NaNO3 0.0216 0.0374 0.0216
Plant biomass (12h incubation) Expression profiling on diverse plant biomasses as carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 0.0193 0.0334 0.0193
Citrus peel 0.0558 0.0966 0.0558
Poplar leaves 0.0373 0.0323 0.0186
Typha leaves 0.0578 0.0615 0.0355
Overwintered Salix leaves 0.0354 0.0308 0.0178
Reed flowering 0.0802 0.0938 0.0542
Tigernut 0.0184 0.0318 0.0184
Energy cane 0.0413 0.0366 0.0211
Guar gum 0.0775 0.0261 0.0151
Apple peels 0.1143 0.0476 0.0275
Cellobiose 0.1486 0.1287 0.0743
Corn stalk 0.1204 0.1718 0.0992
Horse manure 0.4051 0.5861 0.3384
Lignin 0.0561 0.0972 0.0561
Microcrystalline cellulose 0 0 0
Oak leaves 0.2007 0.1781 0.1028
Pectin esterified 0.1149 0.0872 0.0503
Poplar sawdust 0.0259 0.0448 0.0259
Wheat bran 0.1066 0.1053 0.0608
Chlamydomonas reinhardtii 0.0853 0.1018 0.0588
Vertatryl alcohol 0.1361 0.0872 0.0504
Furfural 0.0197 0.0341 0.0197
Autoclaved mycelium 0.09 0.0837 0.0483
Simple and complex carbon and nitrogen sources Expression profiling on diverse complex carbon and nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 0.2557 0.0749 0.0432
Ribose 0 0 0
Mannose 0.4365 0.354 0.2503
Fructose 0.114 0.1543 0.0891
Arabinose 0.1527 0.0653 0.0377
Xylose 0.0918 0.0977 0.0564
Galacturonic acid 0.1148 0.1988 0.1148
Rhamnogalacturonan 0.2305 0.1642 0.0948
Pectin esterified 0.1753 0.1003 0.0579
Polygalacturonic acid 0.3532 0.2143 0.1237
Sodium acetate 0.1923 0.1286 0.0742
No nitrogen 0.1027 0.1032 0.0596
BSA 0.2141 0.1043 0.0602
Glutamine 0.1499 0.0758 0.0438
No phosphate 0.2152 0.2308 0.1333
No carbon 0.1523 0.0506 0.0358
No sulphur 0.2058 0.1183 0.0683
Simple and complex carbon sources (12h incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 0.0193 0.0334 0.0193
Amylose 0.0306 0.053 0.0306
Fucose 0.0178 0.0308 0.0178
Galactose 0.0698 0.1208 0.0698
Lactose 0.1128 0.0346 0.02
Maltose 0.0498 0.0471 0.0272
Mannitol 0.0644 0.0709 0.0409
Rhamnose 0.0794 0.0277 0.016
Sorbitol 0.0415 0.0431 0.0249
Trehalose 0.132 0.127 0.0733
Glycerol 0.0973 0.0157 0.009
Glucuronic acid 0.0704 0.0632 0.0365
Arabinan 0.0451 0.0457 0.0264
Galactan 0.0553 0.0014 0.0008
Galactomannan 0.039 0.0675 0.039
Glucan 0.081 0.0702 0.0405
Xylan 0.0626 0.026 0.015
Xyloglucan 0.1838 0.1501 0.0867
Arabinogalactan 0.0512 0.0886 0.0512
Pectin 0.0699 0.0686 0.0396
Rhamnogalacturonan 0.03 0.052 0.03
Polygalacturonic acid 0.0344 0.0596 0.0344
Mannan 0.1557 0.1106 0.0638
Amylopectin 0.0445 0.0397 0.0229
Inulin 0.1133 0.067 0.0387
BSA 0.1398 0.1267 0.0731
Starvation induced by water agar transfer Expression profiling on starvation induced by transfer to water agar Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Control 0 0 0
2h WAT 0.0335 0.0581 0.0335
4h WAT 0 0 0
8h WAT 0 0 0
16h WAT 0 0 0
24h WAT 0.011 0.019 0.011
WAT hyphal knot -30h 0 0 0
WAT hyphal knot -22h 0 0 0
WAT hyphal knot -15h 0 0 0
WAT hyphal knot -7.5h 0 0 0
WAT hyphal knot 0 0 0
WAT hyphal knot +7.5h 0 0 0
Straw time series Time series expression profiling on straw as a carbon source Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 0.0874 0.0837 0.0483
Straw 3h 0.0158 0.0274 0.0158
Straw 6h 0.1407 0.0879 0.0507
Straw 12h 0.0156 0.027 0.0156
Straw 24h 0.0363 0.0329 0.019
Stress conditions 1 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 0 0 0
Frost (-20C, 30min) 0.0041 0.0071 0.0041
Heat shock (47C, 2h) 0 0 0
High CO2 0 0 0
Drought (4% agar, 12h) 0 0 0
Oxidative (1mM H2O2, 3h) 0 0 0
Acidic (pH4, 3h) 0.0113 0.0195 0.0113
Alkaline (pH9, 3h) 0 0 0
Osmotic (sorbitol 2h) 0 0 0
CongoRed (3h) 0.0438 0.0759 0.0438
Cobalt chloride control 0 0 0
Cobalt chloride 2mM 0.0201 0.0347 0.0201
Stress conditions 2 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 0.0322 0.0558 0.0322
Cold stimulation 0.0077 0.0134 0.0077
Scratched surface 0 0 0
Ca2+, 200mM, 24h 0.3587 0.6213 0.3587
Cu2+, 2mM, 6h 0.1188 0.2057 0.1188
Voriconazole 0.1687 0.1757 0.1015
Trichoderma interaction, early 0 0 0
Trichoderma interaction 60h 0 0 0
Cobalt chloride 2mM repeat 0 0 0
Hypoxia 0 0 0
Hypoxia control 0 0 0
Protoplastation control 0 0 0
Protoplastation 3.5h 0 0 0
Protoplastation 1.5h 0 0 0

General data

Systematic name -
Protein id CopciAB_371582.T0
Description Atypical alpha protein kinase

Annotation summary

0 50 100 150 197

Conserved domains

Analysis Signature accession Signature description InterPro Accession Start End
CDD cd04515 Alpha_kinase - 1 165
Pfam PF02816 Alpha-kinase family IPR004166 55 165

SignalP

Prediction Start End Score
No records

Transmembrane domains

Domain n Start End Length
No records

InterPro

Accession Description
IPR004166 MHCK/EF2 kinase
IPR011009 Protein kinase-like domain superfamily

GO

Go id Term Ontology
GO:0004674 protein serine/threonine kinase activity MF
GO:0005524 ATP binding MF
GO:0006468 protein phosphorylation BP

KEGG

KEGG Orthology
No records

EggNOG

COG category Description
G Atypical alpha protein kinase

CAZy

Class Family Subfamily
No records

Transcription factor

Group
No records

Conservation of CopciAB_371582 across fungi.

Arrow shows the origin of gene family containing CopciAB_371582.

Protein

Sequence id CopciAB_371582.T0
Sequence >CopciAB_371582.T0
MLELFYERCEEKGVDAYLYLSFAKGLLLQEIGKHSPASGQLCDSETDHDGQEVIGIKWLAEERHLSRRIVRFCGT
MQHQTTRTDLAYQTVHTFNHFVYLHTNRQLIFADLQGTPTSVKLASGEVVEGMMLFDPMTHTINHGSGIGDFGQE
GIDTFVEQHTCGPLCRALHLLDSDGSDVNAPKATDTEESEDEAQDND
Length 197

Coding

Sequence id CopciAB_371582.T0
Sequence >CopciAB_371582.T0
ATGCTCGAGCTGTTCTACGAGCGATGTGAAGAGAAGGGTGTCGATGCCTACCTCTATCTTTCCTTCGCGAAGGGC
CTTCTGTTGCAGGAGATTGGAAAACACTCACCTGCTTCTGGCCAGTTATGCGATTCTGAGACAGACCATGATGGA
CAGGAGGTCATCGGTATTAAGTGGCTCGCTGAGGAGCGTCACTTAAGCCGCCGTATTGTGCGGTTCTGTGGCACG
ATGCAGCACCAGACCACCAGGACGGACTTAGCGTATCAAACCGTCCACACCTTCAATCATTTCGTGTACTTACAT
ACAAACCGACAGCTCATTTTTGCTGATTTGCAAGGAACACCGACTTCTGTCAAGCTGGCTAGCGGCGAAGTCGTA
GAGGGAATGATGTTGTTTGATCCTATGACCCATACCATTAATCATGGCTCTGGAATCGGAGATTTTGGACAAGAG
GGCATTGACACCTTTGTTGAGCAACACACCTGTGGACCACTGTGCCGTGCCTTGCACTTGCTCGACTCAGACGGC
TCCGATGTAAATGCCCCTAAAGCAACCGATACTGAGGAGTCAGAAGATGAGGCGCAAGACAACGATTAG
Length 594

Transcript

Sequence id CopciAB_371582.T0
Sequence >CopciAB_371582.T0
ATGCTCGAGCTGTTCTACGAGCGATGTGAAGAGAAGGGTGTCGATGCCTACCTCTATCTTTCCTTCGCGAAGGGC
CTTCTGTTGCAGGAGATTGGAAAACACTCACCTGCTTCTGGCCAGTTATGCGATTCTGAGACAGACCATGATGGA
CAGGAGGTCATCGGTATTAAGTGGCTCGCTGAGGAGCGTCACTTAAGCCGCCGTATTGTGCGGTTCTGTGGCACG
ATGCAGCACCAGACCACCAGGACGGACTTAGCGTATCAAACCGTCCACACCTTCAATCATTTCGTGTACTTACAT
ACAAACCGACAGCTCATTTTTGCTGATTTGCAAGGAACACCGACTTCTGTCAAGCTGGCTAGCGGCGAAGTCGTA
GAGGGAATGATGTTGTTTGATCCTATGACCCATACCATTAATCATGGCTCTGGAATCGGAGATTTTGGACAAGAG
GGCATTGACACCTTTGTTGAGCAACACACCTGTGGACCACTGTGCCGTGCCTTGCACTTGCTCGACTCAGACGGC
TCCGATGTAAATGCCCCTAAAGCAACCGATACTGAGGAGTCAGAAGATGAGGCGCAAGACAACGATTAG
Length 594

Gene

Sequence id CopciAB_371582.T0
Sequence >CopciAB_371582.T0
ATGCTCGAGCTGTTCTACGAGCGATGTGAAGAGAAGGGTGTCGATGCCTACCTCTGTGAGTGCCGTTCTTACTCG
CCCATTCAGTGTTCAGGTTCAGTTCCGCTTATATTCATTCTCACACCTTGTTTAGATCTTTCCTTCGCGAAGGGC
CTTCTGTTGCAGGAGATTGGAAAACACTCACCTGCTTCTGGCCAGTTATGCGATTCTGAGACAGACCATGATGGA
CAGGAGGTCATCGGTATTAAGTGGCTCGCTGAGGAGCGTCACTTAAGCCGCCGTATTGTGCGGTTCTGTGGCACG
ATGCAGCACCAGACCACCAGGACGGACTTAGCGTATCAAACCGTCCACACCTTCAATCATTTCGTGTACTTACAT
ACAAACCGACAGCTCATTTTTGCTGATTTGCAAGGTATGTCCTGACTTACTTCTGCAATCAGGCTATGCACCTAA
ACATACGTCGTTTTAGGAACACCGACTTCTGTCAAGCTGGCTAGCGGCGAAGTCGTAGAGGGAATGATGTTGTTT
GATCCTATGACCCATACCATTAATCAGTATGTGCCCATCCGTCAATCTTTTAATTTACGCCGATCTAATGGGTTT
TTGGGATGTGTTACAGTGGCTCTGGAATCGGAGATTTTGGACAAGAGGGCATTGACACCTTTGTTGAGCAACACA
CCTGTGGACCACTGTGCCGTGCCTTGCACTTGCTCGACTCAGACGGCTCCGATGTAAATGCCCCTAAAGCAACCG
ATACTGAGGAGTCAGAAGATGAGGCGCAAGACAACGATTAG
Length 791