CopciAB_374043
Coprinopsis cinerea A43mutB43mut pab1-1 #326

General data

Systematic name CopciAB_374043 Strain Coprinopsis cinerea Okayama 7
Standard name BUD32 Synonyms 374043
Uniprot id Functional description Lipopolysaccharide kinase (Kdo/WaaP) family
Location scaffold_13:381466..383939 Strand +
Gene length (nt) 2474 Transcript length (nt) 1207
CDS length (nt) 927 Protein length (aa) 308

Reciprocal best hits in model fungi

Strain name Gene / Protein name
Aspergillus nidulans AN2513_pipA
Schizosaccharomyces pombe SPAP27G11.07c
Saccharomyces cerevisiae YGR262C_BUD32
Fusarium graminearum BUD32
Candida albicans BUD32
Neurospora crassa prk-11

Orthologs in mushroom models

Strain name Gene / Protein name Pident E-value Bits
Hypsizygus marmoreus strain 51987-8 Hypma_RDB21769 56.8 4.506E-94 308
Ganoderma sp. 10597 SS1 v1.0 Gansp1_165856 55.5 9.228E-92 301
Schizophyllum commune H4-8 Schco3_2629818 54 2.991E-89 294
Agrocybe aegerita Agrae_CAA7264457 53.3 6.102E-89 293
Lentinula edodes NBRC 111202 Lenedo1_1164106 54.1 2.866E-88 291
Lentinula edodes B17 Lened_B_1_1_7469 61.1 2.911E-85 282
Auricularia subglabra Aurde3_1_1357518 48.4 2.739E-75 254
Pleurotus ostreatus PC15 PleosPC15_2_1079844 57.2 1.985E-71 242
Flammulina velutipes Flave_chr09AA00296 58.5 7.464E-65 223
Lentinula edodes W1-26 v1.0 Lentinedodes1_1669 58 9.298E-63 217
Pleurotus ostreatus PC9 PleosPC9_1_90152 56.3 7.608E-52 185

Expression

Name Summary Attribution Assay type
Carbon metabolism Transcriptional changes along alternative morphogenetic paths of C. cinerea Xie et al. 2020 RNA-Seq
Sample Mean cpm SD cpm Se of mean
sclerotia 28.6992 1.6498 0.9525
oidia 45.9296 6.0054 3.4672
vegetative_mycelium 40.4133 7.023 4.0547
primordia 44.3672 1.9948 1.1517
hyphal_knot 44.5216 7.755 4.4773
young_fruiting_body 31.909 4.7284 2.73
Complex carbon sources 1 (long incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control - glucose 49.3278 6.2025 3.581
Wheat bran 37.9803 4.776 2.7574
Horse manure 50.2268 19.2686 11.1247
Oak leaves 36.9366 11.6233 6.7107
Hay 43.6338 6.7058 3.8716
Corn stalk 45.4973 5.7133 3.2986
Pectine 35.4563 4.4083 2.5451
Microcrystalline cellulose 36.3704 3.071 1.7731
Xylose 38.7596 7.9524 4.5913
Cellobiose 45.2127 3.053 1.7626
Lignin 42.2882 14.4546 8.3453
Apple peels 56.5179 5.2521 3.0323
Coprinopsis challenged with bacteria Transcriptional response to Bacillus subtilis and Escherichia coli. Kombrink et al. 2018 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control 54.9152 2.3574 1.361
Bsubtilis 42.9993 1.412 0.8152
Ecoli 41.8868 6.6872 3.8609
Early development 1 Time series expression profiling of basidiospore and oidium germination Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
BS 0h 26.578 6.7846 3.9171
BS 4h 68.3631 7.3969 4.2706
BS 8h 78.6762 4.7251 2.728
BS 12h 93.9266 21.6975 12.527
Oidia 0 h 13.1962 2.0402 1.1779
Oidia 18 h 53.3146 4.2947 2.4795
Sclerotia 76.4724 3.4228 1.9762
Early development 2 Time series expression profiling of mycelial growth, light induction and fruiting body initiation. Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
DM 12h 32.506 7.2873 4.2073
DM 36h 18.3735 3.4746 2.006
DM 60h 23.8987 2.9782 1.7195
DM 84h 35.7958 7.2131 4.1645
DM 108h 26.6796 2.255 1.3019
DM 132h 29.538 4.7041 2.7159
DM 156hAE 51.7387 12.9348 7.4679
ELI1hAE 56.2908 17.4944 10.1004
ELI2hAE 53.803 23.7922 13.7364
LI2hAE 50.697 21.1366 12.2032
LI6hAE 63.3882 5.486 3.1674
LI12hAE 51.6331 10.4817 6.0516
LI18hAE 56.795 17.9425 10.3591
LI 24hAE 46.2351 9.6968 5.5985
DM 156hAM 38.2327 3.0123 1.7392
ELI1hAM 48.528 6.9645 4.0209
ELI2hAM 54.7403 11.5768 6.6839
LI2hAM 49.3599 17.774 10.2618
LI6hAM 77.1079 2.7227 1.5719
LI12hAM 75.6107 7.4405 4.2958
LI18hAM 79.2121 18.0057 10.3956
LI 24hAM 44.1006 11.1172 6.4185
LI 24hHK 54.3859 15.0046 8.6629
L/D 6h 44.4264 11.6641 6.7343
L/D 12h 44.9151 7.9112 4.5675
L/D 18h 33.8622 0.4069 0.2349
L/D 24h 25.9028 2.2221 1.2829
Fruiting body development RNA-Seq analysis of fruiting body development Krizsan et al. 2019 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Vegetative mycelium 45.0809 9.4813 5.474
Hyphal knots 26.7997 7.6168 4.3976
Primordium 1 30.8804 2.6999 1.5588
Primordium 2 37.2909 5.2917 3.0551
Young Fruiting body cap 41.8838 11.5552 6.6714
Younf fruiting body gill 50.3902 10.2072 5.8931
Young fruiting body stipe 49.4115 6.9105 3.9898
Mature fruiting body cap_gill 46.0938 11.7021 6.7562
Mature fruiting body stipe 85.1556 18.0637 10.4291
Nitrogen sources (12h incubation) Expression profiling on diverse nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Complete medium - control 23.3467 6.6129 3.818
NH4NO3 36.6006 6.1407 3.5453
Proline 27.9434 2.686 1.5508
Tryptophan 38.5199 13.0848 7.5545
Isoleucin 27.4724 2.6949 1.5559
Arginine 23.4374 2.809 1.6218
Metionine 21.3546 5.2647 3.0396
NaNO3 26.2854 2.1974 1.2687
Plant biomass (12h incubation) Expression profiling on diverse plant biomasses as carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 23.3467 6.6129 3.818
Citrus peel 17.72 1.317 0.7604
Poplar leaves 28.601 4.0506 2.3386
Typha leaves 27.0226 7.5715 4.3714
Overwintered Salix leaves 30.2016 3.5238 2.0345
Reed flowering 25.478 6.3737 3.6799
Tigernut 27.3475 1.1762 0.6791
Energy cane 28.4644 2.2339 1.2898
Guar gum 35.6574 4.8907 2.8237
Apple peels 35.6826 12.5814 7.2639
Cellobiose 31.4354 1.9327 1.1158
Corn stalk 44.6613 16.3637 9.4476
Horse manure 37.8591 14.8291 8.5616
Lignin 29.146 2.6001 1.5012
Microcrystalline cellulose 27.5773 3.9482 2.2795
Oak leaves 39.3112 5.321 3.0721
Pectin esterified 50.6087 8.019 4.6298
Poplar sawdust 43.6052 16.0397 9.2605
Wheat bran 61.6738 12.3886 7.1526
Chlamydomonas reinhardtii 27.2528 3.7059 2.1396
Vertatryl alcohol 39.9528 4.9822 2.8765
Furfural 31.7918 4.6163 2.6652
Autoclaved mycelium 29.738 1.9664 1.1353
Simple and complex carbon and nitrogen sources Expression profiling on diverse complex carbon and nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 84.7852 2.1763 1.2565
Ribose 68.2863 2.576 1.4873
Mannose 71.4086 5.2089 3.6832
Fructose 67.0341 2.6291 1.5179
Arabinose 64.732 6.8585 3.9597
Xylose 69.1272 4.7967 2.7694
Galacturonic acid 67.9802 7.9931 4.6148
Rhamnogalacturonan 66.8934 4.3694 2.5227
Pectin esterified 65.0837 4.0808 2.3561
Polygalacturonic acid 61.6623 6.8721 3.9676
Sodium acetate 56.251 4.6155 2.6648
No nitrogen 54.5721 0.6049 0.3492
BSA 56.17 4.7211 2.7257
Glutamine 58.4723 3.0963 1.7876
No phosphate 70.803 4.8165 2.7808
No carbon 56.8071 6.5255 4.6142
No sulphur 56.223 11.3594 6.5584
Simple and complex carbon sources (12h incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 23.3467 6.6129 3.818
Amylose 35.1475 4.3597 2.5171
Fucose 31.9102 1.695 0.9786
Galactose 34.5438 3.2708 1.8884
Lactose 37.7261 3.2542 1.8788
Maltose 35.8893 3.1364 1.8108
Mannitol 37.2906 1.7165 0.991
Rhamnose 34.9234 2.2691 1.3101
Sorbitol 37.3549 2.9679 1.7135
Trehalose 36.9199 4.8151 2.78
Glycerol 32.6852 3.3703 1.9458
Glucuronic acid 43.5698 7.201 4.1575
Arabinan 36.6959 1.9172 1.1069
Galactan 28.2956 3.179 1.8354
Galactomannan 24.9817 7.7359 4.4663
Glucan 24.7133 5.3014 3.0608
Xylan 39.478 2.7797 1.6049
Xyloglucan 42.5749 7.0234 4.0549
Arabinogalactan 33.8407 7.1893 4.1508
Pectin 33.5295 4.9252 2.8436
Rhamnogalacturonan 44.9871 4.5441 2.6236
Polygalacturonic acid 39.8533 2.3353 1.3483
Mannan 39.1583 6.2126 3.5868
Amylopectin 34.0563 1.0244 0.5914
Inulin 22.6402 1.5754 0.9095
BSA 23.4632 2.8403 1.6398
Starvation induced by water agar transfer Expression profiling on starvation induced by transfer to water agar Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Control 23.8987 2.9782 1.7195
2h WAT 67.1149 18.5253 10.6956
4h WAT 50.7857 2.1071 1.2165
8h WAT 58.7179 8.9712 5.1795
16h WAT 75.3311 6.977 4.0282
24h WAT 95.1316 5.9346 3.4263
WAT hyphal knot -30h 30.3917 8.5332 4.9267
WAT hyphal knot -22h 39.9436 6.0545 3.4956
WAT hyphal knot -15h 57.1467 3.5662 2.0589
WAT hyphal knot -7.5h 57.838 0.6571 0.3794
WAT hyphal knot 70.6212 20.2175 11.6726
WAT hyphal knot +7.5h 105.8762 21.7283 12.5448
Straw time series Time series expression profiling on straw as a carbon source Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 38.9477 16.0726 9.2795
Straw 3h 19.2747 3.8124 2.2011
Straw 6h 24.3192 7.1878 4.1499
Straw 12h 25.6146 5.8062 3.3522
Straw 24h 35.5566 5.9788 3.4518
Stress conditions 1 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 53.3946 14.9651 8.6401
Frost (-20C, 30min) 57.6465 3.5084 2.0255
Heat shock (47C, 2h) 16.0732 7.0198 4.0529
High CO2 57.4676 6.7559 3.9005
Drought (4% agar, 12h) 87.6393 6.6111 3.8169
Oxidative (1mM H2O2, 3h) 25.5582 8.8501 5.1096
Acidic (pH4, 3h) 37.5489 8.4365 4.8708
Alkaline (pH9, 3h) 60.9 2.847 1.6437
Osmotic (sorbitol 2h) 56.0638 18.8245 10.8684
CongoRed (3h) 65.3245 7.6678 4.427
Cobalt chloride control 67.1893 11.715 6.7637
Cobalt chloride 2mM 62.9049 16.4763 9.5126
Stress conditions 2 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 100.5336 33.4035 19.2855
Cold stimulation 55.3216 4.5545 2.6296
Scratched surface 37.3674 6.1367 3.543
Ca2+, 200mM, 24h 42.5473 8.7484 5.0509
Cu2+, 2mM, 6h 49.2128 19.0736 11.0121
Voriconazole 64.7086 7.8296 4.5204
Trichoderma interaction, early 43.3289 13.4789 7.7821
Trichoderma interaction 60h 34.6617 7.4595 4.3067
Cobalt chloride 2mM repeat 34.7347 7.4237 4.2861
Hypoxia 89.8193 5.4079 3.1222
Hypoxia control 111.5557 13.4098 7.7421
Protoplastation control 100.403 9.5638 6.7627
Protoplastation 3.5h 50.4645 10.4501 7.3893
Protoplastation 1.5h 35.5989 2.1733 1.5368

General data

Systematic name BUD32
Protein id CopciAB_374043.T0
Description Lipopolysaccharide kinase (Kdo/WaaP) family

Annotation summary

0 100 200 300 308

Conserved domains

Analysis Signature accession Signature description InterPro Accession Start End
Pfam PF06293 Lipopolysaccharide kinase (Kdo/WaaP) family - 186 274

SignalP

Prediction Start End Score
No records

Transmembrane domains

Domain n Start End Length
No records

InterPro

Accession Description
IPR011009 Protein kinase-like domain superfamily
IPR000719 Protein kinase domain
IPR022495 Serine/threonine-protein kinase Bud32
IPR008266 Tyrosine-protein kinase, active site

GO

Go id Term Ontology
GO:0004672 protein kinase activity MF
GO:0005524 ATP binding MF
GO:0006468 protein phosphorylation BP
GO:0004674 protein serine/threonine kinase activity MF

KEGG

KEGG Orthology
K08851

EggNOG

COG category Description
T Lipopolysaccharide kinase (Kdo/WaaP) family

CAZy

Class Family Subfamily
No records

Transcription factor

Group
No records

Conservation of CopciAB_374043 across fungi.

Arrow shows the origin of gene family containing CopciAB_374043.

Protein

Sequence id CopciAB_374043.T0
Sequence >CopciAB_374043.T0
MLSSHSQRPLPASQQLIANAQAISQGAEARIYKTSLTSSNAFSPSTPSAPTTTTAATTTTTTESSQPPAPQSPPI
LLKHRFKKKYRHDVLDVSLTKQRVAGEARALLKCLRSGVNVPQILMVDAAEGILGIEWIDGHSVRQLLPGGEEEE
EEGEEQREEGQQDAPDSIDEAEERLKKEYGVDIDTLLTLIGTELSKLHKADIIHGDLTTSNMMVRRKAGGGGGGG
GGGGAEVLLIDFGLSYTSTLTEDKAVDLYVLERAFASTHPDSERLFGAVLGAYERGLGPKEWHTVKKRLDEVRLR
GRKRSMVG
Length 308

Coding

Sequence id CopciAB_374043.T0
Sequence >CopciAB_374043.T0
ATGCTCTCCTCGCACTCCCAACGGCCCCTCCCGGCATCGCAACAACTCATCGCAAACGCGCAAGCTATCTCGCAG
GGTGCTGAAGCTAGAATCTACAAAACGTCTTTAACTTCTTCGAACGCCTTCTCCCCGTCCACACCCTCGGCTCCA
ACGACGACAACAGCGGCCACCACCACAACGACAACAGAATCATCACAACCCCCAGCACCACAATCCCCTCCCATC
CTCCTCAAACACCGCTTCAAAAAAAAGTACCGACACGACGTGTTGGATGTGTCGTTGACGAAGCAGAGGGTGGCC
GGCGAGGCTCGGGCGTTGTTGAAGTGTTTGCGATCCGGCGTCAACGTCCCGCAAATCCTCATGGTGGACGCAGCG
GAGGGTATCCTCGGTATCGAATGGATAGATGGCCACAGTGTGCGGCAATTACTACCCGGTGGCGAGGAGGAAGAA
GAGGAAGGCGAAGAACAAAGAGAAGAAGGACAGCAAGACGCACCAGACTCGATAGATGAAGCTGAGGAAAGGTTG
AAGAAGGAGTATGGTGTTGATATCGACACCCTCCTAACCCTCATCGGCACCGAACTTTCCAAACTCCACAAAGCG
GATATCATACATGGAGACTTGACCACGTCGAATATGATGGTGCGGCGGAAGGCTGGTGGAGGAGGAGGAGGAGGA
GGAGGAGGAGGAGCTGAAGTGCTCCTAATAGACTTTGGCCTCTCCTACACCTCTACTCTAACCGAAGACAAAGCG
GTCGACTTGTACGTCCTCGAGCGCGCGTTTGCGTCTACGCACCCTGATTCGGAGCGGTTGTTTGGTGCTGTGTTG
GGGGCTTATGAGCGGGGGTTGGGCCCGAAGGAATGGCACACTGTTAAGAAGAGGTTGGATGAAGTGAGGTTGAGA
GGGCGGAAGAGGAGTATGGTTGGGTAG
Length 927

Transcript

Sequence id CopciAB_374043.T0
Sequence >CopciAB_374043.T0
GTTACATCCTTTCCCAGTCCATAAGCTCTACTCGTCGCTCTACTCTTAAGGAGAAAGGCTTTTCTTCGTGGGCTA
TAAAAGCAAATGCTCTCCTCGCACTCCCAACGGCCCCTCCCGGCATCGCAACAACTCATCGCAAACGCGCAAGCT
ATCTCGCAGGGTGCTGAAGCTAGAATCTACAAAACGTCTTTAACTTCTTCGAACGCCTTCTCCCCGTCCACACCC
TCGGCTCCAACGACGACAACAGCGGCCACCACCACAACGACAACAGAATCATCACAACCCCCAGCACCACAATCC
CCTCCCATCCTCCTCAAACACCGCTTCAAAAAAAAGTACCGACACGACGTGTTGGATGTGTCGTTGACGAAGCAG
AGGGTGGCCGGCGAGGCTCGGGCGTTGTTGAAGTGTTTGCGATCCGGCGTCAACGTCCCGCAAATCCTCATGGTG
GACGCAGCGGAGGGTATCCTCGGTATCGAATGGATAGATGGCCACAGTGTGCGGCAATTACTACCCGGTGGCGAG
GAGGAAGAAGAGGAAGGCGAAGAACAAAGAGAAGAAGGACAGCAAGACGCACCAGACTCGATAGATGAAGCTGAG
GAAAGGTTGAAGAAGGAGTATGGTGTTGATATCGACACCCTCCTAACCCTCATCGGCACCGAACTTTCCAAACTC
CACAAAGCGGATATCATACATGGAGACTTGACCACGTCGAATATGATGGTGCGGCGGAAGGCTGGTGGAGGAGGA
GGAGGAGGAGGAGGAGGAGGAGCTGAAGTGCTCCTAATAGACTTTGGCCTCTCCTACACCTCTACTCTAACCGAA
GACAAAGCGGTCGACTTGTACGTCCTCGAGCGCGCGTTTGCGTCTACGCACCCTGATTCGGAGCGGTTGTTTGGT
GCTGTGTTGGGGGCTTATGAGCGGGGGTTGGGCCCGAAGGAATGGCACACTGTTAAGAAGAGGTTGGATGAAGTG
AGGTTGAGAGGGCGGAAGAGGAGTATGGTTGGGTAGGCGAGGTGTTTCTGTGTTATGGTTGGGTAGACGAGGTGT
TTCTGTGTATGGGGTTGGGTAGACGAGCTGGTTTCTGTTTTATCTATTTTTTTTTCGGAGCGAAACGTTGTAGTA
TGGACGAGTCGGATGTGTATGTAGTGCAAAACGGTTACAGCGTATGGGTGTAGCGCAATGTACAAAAACGTTGTA
TGTGTGT
Length 1207

Gene

Sequence id CopciAB_374043.T0
Sequence >CopciAB_374043.T0
GTTACATCCTTTCCCAGTCCATAAGCTCTACTCGTCGCTCTACTCTTAAGGAGAAAGGCTTTTCTTCGTGGGCTA
TAAAAGCAAATGCTCTCCTCGCACTCCCAACGGCCCCTCCCGGCATCGCAACAACTCATCGCAAACGCGCAAGCT
ATCTCGCAGGGTGCTGAAGCTGCAAGTCCTTCCTCCTTGTCGTTTAAGGATGCGTTGAATAGCTTATACTCAGGG
ATCCCGTTATACCACTTTACTTGCCTTGGTGTCGTCACCCTGGTTCTGTCTATCGACAAACGATAAACGTCCGGG
GCGAATCCTCCTATCCCATGACCGTCGATCATAACAGAGAATCTACAAAACGTCTTTAACTTCTTCGAACGCCTT
CTCCCCGTCCACACCCTCGGCTCCAACGACGACAACAGCGGCCACCACCACAACGACAACAGAATCATCACAACC
CCCAGCACCACAATCCCCTCCCATCCTCCTCAAACACCGCTTCAAAAAAAAGTACCGACACGACGTGTTGGATGT
GTCGTTGACGAAGCAGAGGGTGGCCGGCGAGGCTCGGGCGTTGTTGAAGTGTTTGCGGTTAGTGCTCAATCGCGG
TTTCTCTTTCTCTTTCTTTCGTTCTTTGGCCATTCTTCGTTGGCGGTCCCGCTTCGATGATCTCCTCTGTTCGAC
GACGACTCCCCATCTGCTCAACGCCTACTACCTCAGTCGCTATCCGTTATGAGGGAACCAGTGACGACTCGCTAA
CCCCCACCCCCATCGTCATCTAGATCCGGCGTCAACGTCCCGCAAATCCTCATGGTGGACGCAGCGGAGGGTATC
CTCGGTATCGAATGGATAGATGGCCACAGTGTGCGGCAATTACTACCCGGTGGCGAGGAGGAAGAAGAGGAAGGC
GAAGAACAAAGAGAAGGTGAGGAATAGTTTACTGTTACACGCCTTTCGAGGTGGATGGAACTAACGCCGGGGGTT
GAAATCGAAGAAGGACAGCAAGACGCACCAGACTCGATAGATGAAGCTGAGGAAAGGTTGAAGAAGGAGTATGGT
GTTGATATCGGTGTGTGTTACTTCTTTCCTTCCATCACGTCCTCTCGAACTTACCACGAAATCCATCTCTACCAC
GCCCACCAAATCACGACCCACAGACACCCTCCTAACCCTCATCGGCACCGAACTTTCCAAACTCCACAAAGCGGA
TATCATACATGGAGACTTGACCACGTCGAATATGATGGTGCGGCGGAAGGCTGGTGGAGGAGGAGGAGGAGGAGG
AGGAGGAGGAGCTGAAGTGGTACGTAGTTTCTCTGCATGGTCGTTTGTGCCAGTTATTCTCTTCTTGGTCATTAT
CTTCTCTTCTCAGTTTGCCCCCGTCCCCGTCCTTGTCCACTCTTTCGTTATCCTTCCATCTTCTGCCCTCGCCCT
CTGCCCTCCTCTCCTCTCCCGTCCCTATCTCTACATCTCTACATACAATCCGGCTCCCCCTAACACCCTCCCTCT
CCTCCCCAACAGCTCCTAATAGACTTTGGCCTCTCCTACACCTCTACTCTAACCGAAGACAAAGCGGTCGACTTG
TACGTCCTCGAGCGCGCGTTTGCGTCTACGCACCCTGATTCGGAGCGGTTGTTTGGTGCTGTGTTGGGGGCTTAT
GAGCGGGGGTTGGGTGAGTGTTTTTTTATGTTTTTTTTTTTTTTTGTGGATTCTATTTTTTCTGGATTTAATTGA
TTTTGGGGCTCGGGGGGTTTGAGGTGCGAGTTTTGGAAATGATTGGGTTTGAAAAAAAATCATGGGGGTTTGACG
AGCGTAAATCACGGGGTTTGACGAGCGTGGCGTTGTCCTCGTTGCGAATTCTTGGGCTCTTATGTTCGCGTCCGG
GTCTAGGATCCGGATTTATTTTGGTGACGCGTTGTGATCGTTTGTCCGTTTCTCTTGGAGCGAACTGGGGTGGGT
GGGTAGGTGGGGTGGGTGGGTTGGAGGATGGGTTGACGTGCTTCGTGCTCCTGGACCTTGACTCGATGAACGACG
ATCGCCTGAACCGCGAACGACCTAACGGACTAGCGCTTTCTGAATTTAAAGTCTCGCTAACCCTCTACATTCAAA
CTCCCTCGAACCCCTCTACATTCGAACTCTCTCTAAAACCCGCAGTCGTAAACGGACTGACTAACAAACCCTCCT
TTTTCTTTGGATGTACATTGTAGGCCCGAAGGAATGGCACACTGTTAAGAAGAGGTTGGATGAAGTGAGGTTGAG
AGGGCGGAAGAGGAGTATGGTTGGGTAGGCGAGGTGTTTCTGTGTTATGGTTGGGTAGACGAGGTGTTTCTGTGT
ATGGGGTTGGGTAGACGAGCTGGTTTCTGTTTTATCTATTTTTTTTTCGGAGCGAAACGTTGTAGTATGGACGAG
TCGGATGTGTATGTAGTGCAAAACGGTTACAGCGTATGGGTGTAGCGCAATGTACAAAAACGTTGTATGTGTGT
Length 2474