CopciAB_429744
Coprinopsis cinerea A43mutB43mut pab1-1 #326

General data

Systematic name CopciAB_429744 Strain Coprinopsis cinerea Okayama 7
Standard name tmk1 Synonyms 429744
Uniprot id Functional description CMGC MAPK protein kinase
Location scaffold_1:2430502..2432250 Strand -
Gene length (nt) 1749 Transcript length (nt) 1207
CDS length (nt) 1092 Protein length (aa) 363

Reciprocal best hits in model fungi

Strain name Gene / Protein name

Orthologs in mushroom models

Strain name Gene / Protein name Pident E-value Bits
Hypsizygus marmoreus strain 51987-8 Hypma_RDB29560 94.4 2.101E-236 722
Agrocybe aegerita Agrae_CAA7266567 91 1.01E-234 717
Pleurotus eryngii ATCC 90797 Pleery1_1441362 92.5 1.171E-232 711
Pleurotus ostreatus PC15 PleosPC15_2_1053841 92.5 9.959E-233 711
Pleurotus ostreatus PC9 PleosPC9_1_53880 92.5 9.748E-233 711
Flammulina velutipes Flave_chr08AA00827 90.9 1.865E-226 693
Lentinula edodes NBRC 111202 Lenedo1_263629 87.7 4.215E-218 669
Lentinula edodes W1-26 v1.0 Lentinedodes1_2340 87.7 4.182E-218 669
Lentinula edodes B17 Lened_B_1_1_2210 87.8 1.543E-209 644
Ganoderma sp. 10597 SS1 v1.0 Gansp1_113395 83.1 5.23E-206 634
Grifola frondosa Grifr_OBZ79590 83.3 2.824E-204 629

Expression

Name Summary Attribution Assay type
Carbon metabolism Transcriptional changes along alternative morphogenetic paths of C. cinerea Xie et al. 2020 RNA-Seq
Sample Mean cpm SD cpm Se of mean
sclerotia 261.23 40.4037 23.3271
oidia 220.9134 19.1312 11.0454
vegetative_mycelium 339.7712 41.443 23.9271
primordia 21.4912 3.1292 1.8066
hyphal_knot 202.9761 30.4118 17.5582
young_fruiting_body 13.3585 5.2141 3.0103
Complex carbon sources 1 (long incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control - glucose 366.4976 75.3011 43.4751
Wheat bran 368.9911 61.9818 35.7852
Horse manure 374.1829 74.9043 43.246
Oak leaves 445.1523 104.4813 60.3223
Hay 275.8436 50.5822 29.2036
Corn stalk 355.6331 50.759 29.3057
Pectine 302.5749 49.4353 28.5415
Microcrystalline cellulose 263.3008 26.0528 15.0416
Xylose 531.8891 82.8136 47.8125
Cellobiose 489.1415 8.2551 4.7661
Lignin 234.6027 63.9383 36.9148
Apple peels 351.7329 10.898 6.292
Coprinopsis challenged with bacteria Transcriptional response to Bacillus subtilis and Escherichia coli. Kombrink et al. 2018 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Control 352.8939 32.0322 18.4938
Bsubtilis 365.3809 28.835 16.6479
Ecoli 381.1874 21.1462 12.2087
Early development 1 Time series expression profiling of basidiospore and oidium germination Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
BS 0h 2.4074 0.1216 0.0702
BS 4h 16.536 3.6092 2.0838
BS 8h 60.123 7.9988 4.6181
BS 12h 102.5943 11.8312 6.8307
Oidia 0 h 11.486 4.0615 2.3449
Oidia 18 h 99.0764 18.6906 10.791
Sclerotia 249.211 32.4976 18.7625
Early development 2 Time series expression profiling of mycelial growth, light induction and fruiting body initiation. Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
DM 12h 136.1422 15.762 9.1002
DM 36h 146.3547 6.3622 3.6732
DM 60h 190.5143 15.9315 9.1981
DM 84h 190.8206 10.2262 5.9041
DM 108h 190.6982 18.1691 10.4899
DM 132h 166.7163 11.9387 6.8928
DM 156hAE 226.6157 42.3913 24.4747
ELI1hAE 165.562 13.7739 7.9524
ELI2hAE 144.6606 9.0654 5.2339
LI2hAE 140.9735 6.2673 3.6184
LI6hAE 176.2167 18.7312 10.8145
LI12hAE 142.6518 19.2794 11.131
LI18hAE 163.9826 38.9435 22.484
LI 24hAE 179.6726 17.9712 10.3757
DM 156hAM 189.3215 12.1278 7.002
ELI1hAM 205.3362 51.6641 29.8283
ELI2hAM 193.4613 9.9411 5.7395
LI2hAM 173.345 34.2906 19.7977
LI6hAM 221.8312 24.9538 14.4071
LI12hAM 213.7831 12.4548 7.1908
LI18hAM 185.9518 13.3341 7.6984
LI 24hAM 165.682 28.772 16.6115
LI 24hHK 169.2539 35.1602 20.2998
L/D 6h 126.0397 16.2849 9.4021
L/D 12h 98.2536 13.4405 7.7599
L/D 18h 79.1005 16.4153 9.4774
L/D 24h 70.6962 8.3264 4.8073
Fruiting body development RNA-Seq analysis of fruiting body development Krizsan et al. 2019 RNA-Seq
Sample Mean cpm SD cpm Se of mean
Vegetative mycelium 170.9796 27.0625 15.6245
Hyphal knots 134.9956 23.1259 13.3517
Primordium 1 79.4372 28.219 16.2922
Primordium 2 89.0276 17.4281 10.0621
Young Fruiting body cap 24.6955 2.0148 1.1632
Younf fruiting body gill 20.8469 5.199 3.0016
Young fruiting body stipe 29.8186 16.8518 9.7294
Mature fruiting body cap_gill 8.9053 1.4056 0.8115
Mature fruiting body stipe 38.1542 14.6351 8.4496
Nitrogen sources (12h incubation) Expression profiling on diverse nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Complete medium - control 220.2874 30.4363 17.5724
NH4NO3 194.666 6.3522 3.6674
Proline 219.1669 52.3563 30.2279
Tryptophan 220.5835 111.7339 64.5096
Isoleucin 189.5949 2.079 1.2003
Arginine 198.4469 30.0923 17.3738
Metionine 217.6026 12.648 7.3023
NaNO3 209.0448 18.4873 10.6736
Plant biomass (12h incubation) Expression profiling on diverse plant biomasses as carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 220.2874 30.4363 17.5724
Citrus peel 184.1458 7.6064 4.3916
Poplar leaves 150.5171 13.9502 8.0542
Typha leaves 166.7123 38.4262 22.1853
Overwintered Salix leaves 181.4734 20.906 12.0701
Reed flowering 203.3134 27.5765 15.9213
Tigernut 209.81 4.2181 2.4353
Energy cane 203.7 18.9562 10.9444
Guar gum 255.3994 70.5275 40.7191
Apple peels 243.8353 48.324 27.8998
Cellobiose 225.3974 29.2839 16.9071
Corn stalk 204.802 2.0677 1.1938
Horse manure 210.5115 17.0356 9.8355
Lignin 242.3699 163.2904 94.2757
Microcrystalline cellulose 265.517 92.8358 53.5988
Oak leaves 196.4152 15.5941 9.0033
Pectin esterified 237.7107 18.7636 10.8332
Poplar sawdust 206.8883 58.9232 34.0193
Wheat bran 209.2825 17.3624 10.0242
Chlamydomonas reinhardtii 181.3641 10.6629 6.1562
Vertatryl alcohol 116.0437 18.5817 10.7282
Furfural 45.6057 14.1519 8.1706
Autoclaved mycelium 173.1784 27.8644 16.0875
Simple and complex carbon and nitrogen sources Expression profiling on diverse complex carbon and nitrogen sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 406.6685 18.8057 10.8575
Ribose 324.9336 15.8766 9.1663
Mannose 401.3729 11.7557 8.3125
Fructose 358.2694 16.2859 9.4027
Arabinose 328.808 1.508 0.8706
Xylose 355.5947 25.3412 14.6308
Galacturonic acid 331.1733 8.1996 4.734
Rhamnogalacturonan 336.9682 14.3541 8.2873
Pectin esterified 319.332 3.7472 2.1634
Polygalacturonic acid 343.3883 8.4597 4.8842
Sodium acetate 345.917 19.5939 11.3125
No nitrogen 352.9103 15.0689 8.7001
BSA 338.576 8.5918 4.9605
Glutamine 306.5683 7.9011 4.5617
No phosphate 289.2396 11.0366 6.372
No carbon 330.4486 9.9553 7.0394
No sulphur 453.6941 55.233 31.8888
Simple and complex carbon sources (12h incubation) Expression profiling on diverse complex carbon sources. Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose - control 220.2874 30.4363 17.5724
Amylose 208.0487 4.425 2.5548
Fucose 203.7158 13.5777 7.8391
Galactose 217.6148 14.4855 8.3632
Lactose 181.518 16.7914 9.6945
Maltose 165.0663 29.4978 17.0306
Mannitol 138.8992 21.0247 12.1386
Rhamnose 174.4718 18.4446 10.649
Sorbitol 194.6158 20.732 11.9696
Trehalose 222.606 27.4703 15.86
Glycerol 226.6151 57.1161 32.976
Glucuronic acid 219.5172 28.7661 16.6081
Arabinan 214.638 44.0086 25.4084
Galactan 196.4488 15.6998 9.0643
Galactomannan 244.0386 17.7291 10.2359
Glucan 248.2308 52.6232 30.382
Xylan 166.2865 27.0793 15.6343
Xyloglucan 207.2855 10.7146 6.1861
Arabinogalactan 224.194 11.1194 6.4198
Pectin 188.3602 16.2725 9.3949
Rhamnogalacturonan 147.8944 2.9665 1.7127
Polygalacturonic acid 191.0254 27.2815 15.751
Mannan 178.3175 11.1969 6.4645
Amylopectin 203.1383 26.5209 15.3119
Inulin 195.6265 20.2938 11.7166
BSA 183.6264 27.0652 15.6261
Starvation induced by water agar transfer Expression profiling on starvation induced by transfer to water agar Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Control 190.5143 15.9315 9.1981
2h WAT 178.7107 0.8343 0.4817
4h WAT 189.8892 37.8859 21.8735
8h WAT 137.9479 5.5 3.1754
16h WAT 202.3627 16.1734 9.3377
24h WAT 185.3322 2.6477 1.5287
WAT hyphal knot -30h 113.0845 20.2255 11.6772
WAT hyphal knot -22h 120.7862 3.9346 2.2716
WAT hyphal knot -15h 139.2254 7.6684 4.4273
WAT hyphal knot -7.5h 197.8141 18.63 10.756
WAT hyphal knot 181.4396 14.8325 8.5635
WAT hyphal knot +7.5h 233.8802 17.2609 9.9656
Straw time series Time series expression profiling on straw as a carbon source Hegedus et al. RNA-Seq
Sample Mean cpm SD cpm Se of mean
Glucose control 260.4725 43.3167 25.0089
Straw 3h 263.8545 84.9949 49.0718
Straw 6h 201.7623 42.3427 24.4466
Straw 12h 175.9167 10.9342 6.3128
Straw 24h 310.3343 142.8656 82.4835
Stress conditions 1 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 218.0348 33.1901 19.1623
Frost (-20C, 30min) 132.199 10.2544 5.9204
Heat shock (47C, 2h) 38.2498 2.1732 1.2547
High CO2 250.5191 28.3297 16.3562
Drought (4% agar, 12h) 229.8523 48.9904 28.2846
Oxidative (1mM H2O2, 3h) 175.277 7.2627 4.1931
Acidic (pH4, 3h) 207.7422 6.4222 3.7078
Alkaline (pH9, 3h) 239.747 20.9646 12.1039
Osmotic (sorbitol 2h) 134.09 48.0983 27.7695
CongoRed (3h) 236.0937 29.9905 17.315
Cobalt chloride control 204.3165 49.2755 28.4492
Cobalt chloride 2mM 248.0639 52.8305 30.5017
Stress conditions 2 Expression profiling under various stress conditions Hegedus et al. QuantSeq
Sample Mean cpm SD cpm Se of mean
Flask control 159.312 89.6163 51.74
Cold stimulation 223.8217 41.0715 23.7126
Scratched surface 97.2391 6.0748 3.5073
Ca2+, 200mM, 24h 334.2076 30.0892 17.372
Cu2+, 2mM, 6h 219.5672 49.2997 28.4632
Voriconazole 236.3403 1.6179 0.9341
Trichoderma interaction, early 164.7395 9.0771 5.2406
Trichoderma interaction 60h 74.7598 48.6334 28.0785
Cobalt chloride 2mM repeat 95.8367 9.487 5.4773
Hypoxia 185.3648 7.6042 4.3903
Hypoxia control 151.4629 32.3911 18.701
Protoplastation control 136.7447 26.8379 18.9773
Protoplastation 3.5h 109.4207 3.1723 2.2431
Protoplastation 1.5h 163.8201 8.1057 5.7316

General data

Systematic name tmk1
Protein id CopciAB_429744.T0
Description CMGC MAPK protein kinase

Annotation summary

0 100 200 300 363

Conserved domains

Analysis Signature accession Signature description InterPro Accession Start End
CDD cd07849 STKc_ERK1_2_like - 13 358
Pfam PF00069 Protein kinase domain IPR000719 19 314

SignalP

Prediction Start End Score
No records

Transmembrane domains

Domain n Start End Length
No records

InterPro

Accession Description
IPR017441 Protein kinase, ATP binding site
IPR008271 Serine/threonine-protein kinase, active site
IPR003527 Mitogen-activated protein (MAP) kinase, conserved site
IPR000719 Protein kinase domain
IPR011009 Protein kinase-like domain superfamily

GO

Go id Term Ontology
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP
GO:0004707 MAP kinase activity MF

KEGG

KEGG Orthology
K04371

EggNOG

COG category Description
T CMGC MAPK protein kinase

CAZy

Class Family Subfamily
No records

Transcription factor

Group
No records

Conservation of CopciAB_429744 across fungi.

Arrow shows the origin of gene family containing CopciAB_429744.

Protein

Sequence id CopciAB_429744.T0
Sequence >CopciAB_429744.T0
MSSRRNAKPLPKFEIGDDYQLLHAIGEGAYGTVAAALHKPTGRQVAIKKVLPFDHTLFCLRTLRELKLLKFFSET
CVNENIISILDIIKPPSLEEFKEIYFIQELMQTDLHRVIRTQHLTDDHCQYFVYQTLRALKSIHSADIVHRDLKP
ANLLLNANCDLKVCDFGLARSVKTSVPAGKEVGLMTEYVATRWYRAPEIMLSFKMYTKAIDIWAVGCILAELLTG
RPLFPGRDYSHQLDLILDVIGTPSLDEFYAITSRRSRDYIRALPIRKKRPFPTLFPHASQEAIDFLTKTLTFDPK
KRMTVDEALEHPYLAAYHDPEDEPVVTPLSPDYFEFDMHKDDLSKDQLKELLYQEVLSFTPSI
Length 363

Coding

Sequence id CopciAB_429744.T0
Sequence >CopciAB_429744.T0
ATGTCTTCTCGTAGGAACGCAAAACCTCTCCCAAAGTTCGAAATCGGTGACGATTATCAGCTACTGCATGCGATT
GGAGAAGGCGCATACGGGACAGTCGCTGCTGCTCTTCACAAGCCGACAGGGCGCCAGGTCGCCATAAAGAAAGTC
CTCCCTTTCGACCATACATTGTTTTGCCTCCGAACCCTTCGAGAGCTCAAGTTGCTCAAGTTCTTCTCGGAGACT
TGCGTCAACGAAAATATTATTTCAATCCTGGATATTATCAAGCCTCCTTCCCTTGAAGAGTTCAAGGAGATATAC
TTTATCCAGGAATTGATGCAGACTGACTTGCACCGTGTTATCCGTACTCAGCACCTCACGGACGACCACTGCCAA
TACTTCGTTTACCAAACACTCCGGGCGCTCAAATCAATCCACAGCGCCGACATCGTCCACCGTGACCTCAAACCT
GCTAACCTTCTTCTCAATGCCAATTGTGACCTGAAGGTTTGTGACTTTGGCCTGGCTCGCAGCGTGAAGACCAGC
GTCCCCGCCGGGAAAGAAGTCGGTTTGATGACGGAGTACGTCGCCACTCGGTGGTACCGCGCTCCCGAGATCATG
TTGTCCTTCAAGATGTACACCAAGGCAATTGACATCTGGGCTGTGGGCTGTATTCTTGCCGAACTTCTCACTGGA
AGGCCCTTGTTCCCGGGTCGTGATTACAGCCATCAACTCGACCTGATCCTCGATGTGATTGGCACGCCCAGTCTC
GATGAGTTCTATGCTATTACTTCGCGGAGGTCTCGCGACTATATTCGTGCACTTCCCATCCGCAAGAAACGCCCT
TTCCCCACACTTTTCCCGCACGCCTCGCAGGAAGCTATCGATTTCTTAACCAAGACCTTGACCTTTGATCCAAAG
AAACGAATGACTGTGGACGAGGCCTTGGAGCACCCTTATCTCGCGGCTTATCACGACCCCGAAGATGAGCCTGTC
GTGACACCCCTTAGCCCGGACTACTTCGAATTCGACATGCACAAGGACGATCTCAGCAAAGACCAGCTGAAAGAG
CTCCTCTACCAGGAAGTTTTGTCTTTCACCCCTTCTATCTAG
Length 1092

Transcript

Sequence id CopciAB_429744.T0
Sequence >CopciAB_429744.T0
AAGTCCCTCATCGACCATCCCCCGGTGGGTTTCTGGTGTACTAAGAATGTCTTCTCGTAGGAACGCAAAACCTCT
CCCAAAGTTCGAAATCGGTGACGATTATCAGCTACTGCATGCGATTGGAGAAGGCGCATACGGGACAGTCGCTGC
TGCTCTTCACAAGCCGACAGGGCGCCAGGTCGCCATAAAGAAAGTCCTCCCTTTCGACCATACATTGTTTTGCCT
CCGAACCCTTCGAGAGCTCAAGTTGCTCAAGTTCTTCTCGGAGACTTGCGTCAACGAAAATATTATTTCAATCCT
GGATATTATCAAGCCTCCTTCCCTTGAAGAGTTCAAGGAGATATACTTTATCCAGGAATTGATGCAGACTGACTT
GCACCGTGTTATCCGTACTCAGCACCTCACGGACGACCACTGCCAATACTTCGTTTACCAAACACTCCGGGCGCT
CAAATCAATCCACAGCGCCGACATCGTCCACCGTGACCTCAAACCTGCTAACCTTCTTCTCAATGCCAATTGTGA
CCTGAAGGTTTGTGACTTTGGCCTGGCTCGCAGCGTGAAGACCAGCGTCCCCGCCGGGAAAGAAGTCGGTTTGAT
GACGGAGTACGTCGCCACTCGGTGGTACCGCGCTCCCGAGATCATGTTGTCCTTCAAGATGTACACCAAGGCAAT
TGACATCTGGGCTGTGGGCTGTATTCTTGCCGAACTTCTCACTGGAAGGCCCTTGTTCCCGGGTCGTGATTACAG
CCATCAACTCGACCTGATCCTCGATGTGATTGGCACGCCCAGTCTCGATGAGTTCTATGCTATTACTTCGCGGAG
GTCTCGCGACTATATTCGTGCACTTCCCATCCGCAAGAAACGCCCTTTCCCCACACTTTTCCCGCACGCCTCGCA
GGAAGCTATCGATTTCTTAACCAAGACCTTGACCTTTGATCCAAAGAAACGAATGACTGTGGACGAGGCCTTGGA
GCACCCTTATCTCGCGGCTTATCACGACCCCGAAGATGAGCCTGTCGTGACACCCCTTAGCCCGGACTACTTCGA
ATTCGACATGCACAAGGACGATCTCAGCAAAGACCAGCTGAAAGAGCTCCTCTACCAGGAAGTTTTGTCTTTCAC
CCCTTCTATCTAGGACATGTTCTCTGTACTGCATCTAATGATTGCTGTGATATATACATCGATAATTGTATTTGA
ATACCGG
Length 1207

Gene

Sequence id CopciAB_429744.T0
Sequence >CopciAB_429744.T0
AAGTCCCTCATCGACCATCCCCCGGTGGGTTTCTGGTGTACTAAGAATGTCTTCTCGTAGGAACGCAAAACCTCT
CCCAAAGTTCGAAATCGGTACGGCTATCTCCTCCACAATCTGGTACATCGCTTCACCGCCCATATTTACTAGGTG
ACGATTATCAGCTACTGCATGCGATTGGAGAAGGCGCATACGGGACAGTCGCTGCTGCTCTTCACAAGCCGACAG
GGCGCCAGGTCGCCATAAAGAAAGTCCTCCCTTTCGACCATACATTGTTTTGCCTCCGAACCCTTCGAGAGCTCA
AGTTGCTCAAGTTCTTCTCGGAGACTTGCGTCAACGAAAATGTAGATCCAGCTCCATCGTGCACCCTGTTACCTG
CTGATGGCTTGCCAGATTATTTCAATCCTGGATATTATCAAGCCTCCTTCCCTTGAAGAGTTCAAGGAGATATAC
TGTAAGCTCGACGCCCGTACATCGTCCCTCAGTTCCGATTCCATGCCTTCCCTCAGTTATCCAGGAATTGATGCA
GACTGACTTGCACCGTGTTATCCGTACTCAGCACCTCACGGACGACCACTGCCAAGTATGTCGCTTTCAATGACC
CGTTCTCAATACACTACTGACCGTCTGTGTAGTACTTCGTTTACCAAACACTCCGGGCGCTCAAATCAATCCACA
GCGCCGACATCGTCCACCGTGACCTCAAACCTGCTAACCTTCTTCTCAATGCCAATTGTGACCTGAAGGTTTGTG
ACTTTGGCCTGGCTCGCAGCGTGAAGACCAGCGTCCCCGCCGGGAAAGAAGTCGGTTTGATGACGGAGTACGTCG
CCACTCGGTGGTACCGCGCTCCCGAGATCATGTTGTCCTTCAAGATGTACACCAAGGTAGGCGCCAGAAGTACGT
GTCGTTGCTCCCGCTGGCTGAAATGTTGCCTAGGCAATTGACATCTGGGCTGTGGGCTGTATTCTTGCCGAACTT
CTCACTGGAAGGCCCTTGTTCCCGGGTCGTGATTACAGCCATCAACTCGACCTGATCCTCGATGTGATTGGTTAG
TTTTGCACGTTCTTTTGTCTCAAGATGGGATGTGCTAATTCCTATGCAGGCACGCCCAGTCTCGATGAGTTCTAT
GCTATTACTTCGCGGAGGTCTCGCGACTATATTCGTGCACTTCCCATCCGCAAGAAACGCCCTTTCCCCACACTT
TTCCCGCACGCCTCGCAGGAAGCTATCGATTTCTTAACCAAGACCTTGGTCAGTGCCCATTACCACGATAATATA
TTGCAAATCGTATTTACACGCCATTCACCCTGCAGACCTTTGATCCAAAGAAACGAATGACTGTGGACGAGGCCT
TGGAGCACCCTTATCTCGCGGCTTATGTACGTATTGATCGACCTTCCTGTCGATCCTGCAGGACCCTGATCAAAA
TCTAGCACGACCCCGAAGATGAGCCTGTCGTGACACCCCTTAGCCCGGACTACTTCGAATTCGACAGTAAGTCTT
TGCGAGTGGCAAGCTATACGCTGACTTTGACACCTCTCGTCATCAGTGCACAAGGACGATCTCAGCAAAGACCAG
CTGAAAGGTGCGCGCGAGTTCGACATACCATCCACTGCTGCGTAACTGACCCCCCGAGTAGAGCTCCTCTACCAG
GAAGTTTTGTCTTTCACCCCTTCTATCTAGGACATGTTCTCTGTACTGCATCTAATGATTGCTGTGATATATACA
TCGATAATTGTATTTGAATACCGG
Length 1749