|
Carbon metabolism |
Transcriptional changes along alternative morphogenetic paths of C. cinerea |
Xie et al. 2020
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
sclerotia |
3.237 |
0.3554 |
0.2052 |
oidia |
7.823 |
1.8134 |
1.0469 |
vegetative_mycelium |
6.7622 |
1.1997 |
0.6926 |
primordia |
7.5056 |
0.8234 |
0.4754 |
hyphal_knot |
5.922 |
1.0002 |
0.5775 |
young_fruiting_body |
3.5351 |
0.2992 |
0.1728 |
|
|
Complex carbon sources 1 (long incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control - glucose |
5.2798 |
1.5983 |
0.9228 |
Wheat bran |
18.5387 |
7.1271 |
4.1148 |
Horse manure |
8.9147 |
2.2016 |
1.2711 |
Oak leaves |
2.3437 |
0.6367 |
0.3676 |
Hay |
11.6939 |
2.7583 |
1.5925 |
Corn stalk |
9.2731 |
0.6863 |
0.3962 |
Pectine |
7.4692 |
0.3957 |
0.2285 |
Microcrystalline cellulose |
6.3358 |
1.4505 |
0.8374 |
Xylose |
8.9698 |
2.9546 |
1.7058 |
Cellobiose |
12.8451 |
1.0564 |
0.6099 |
Lignin |
6.9676 |
0.5593 |
0.3229 |
Apple peels |
4.4971 |
0.5035 |
0.2907 |
|
|
Coprinopsis challenged with bacteria |
Transcriptional response to Bacillus subtilis and Escherichia coli. |
Kombrink et al. 2018
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
4.1227 |
0.6431 |
0.3713 |
Bsubtilis |
3.2462 |
0.3423 |
0.1976 |
Ecoli |
1.7854 |
0.2769 |
0.1598 |
|
|
Early development 1 |
Time series expression profiling of basidiospore and oidium germination |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
BS 0h |
1.0627 |
0.3896 |
0.2249 |
BS 4h |
2.1222 |
0.4281 |
0.2472 |
BS 8h |
2.745 |
0.8215 |
0.4743 |
BS 12h |
3.1249 |
0.8632 |
0.4984 |
Oidia 0 h |
9.8948 |
2.2246 |
1.2844 |
Oidia 18 h |
1.8253 |
0.5737 |
0.3312 |
Sclerotia |
3.1632 |
2.0106 |
1.1608 |
|
|
Early development 2 |
Time series expression profiling of mycelial growth, light induction and fruiting body initiation. |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
DM 12h |
1.343 |
0.2261 |
0.1306 |
DM 36h |
0.8188 |
0.3033 |
0.1751 |
DM 60h |
1.4695 |
0.5886 |
0.3398 |
DM 84h |
2.0056 |
0.3284 |
0.1896 |
DM 108h |
0.7977 |
0.2332 |
0.1346 |
DM 132h |
1.6273 |
0.6189 |
0.3573 |
DM 156hAE |
1.5116 |
0.381 |
0.22 |
ELI1hAE |
1.5063 |
0.6466 |
0.3733 |
ELI2hAE |
1.147 |
0.739 |
0.4266 |
LI2hAE |
1.582 |
0.5339 |
0.3083 |
LI6hAE |
2.6628 |
0.8267 |
0.4773 |
LI12hAE |
2.2329 |
1.8802 |
1.0855 |
LI18hAE |
1.6604 |
0.5881 |
0.3395 |
LI 24hAE |
1.3277 |
0.4653 |
0.2686 |
DM 156hAM |
1.7555 |
0.0483 |
0.0279 |
ELI1hAM |
2.0419 |
0.2232 |
0.1288 |
ELI2hAM |
1.8338 |
0.2505 |
0.1446 |
LI2hAM |
1.558 |
0.8452 |
0.488 |
LI6hAM |
1.505 |
0.3712 |
0.2143 |
LI12hAM |
3.018 |
0.4479 |
0.2586 |
LI18hAM |
2.6271 |
1.0313 |
0.5954 |
LI 24hAM |
1.4234 |
0.5999 |
0.3464 |
LI 24hHK |
2.9442 |
0.1752 |
0.1011 |
L/D 6h |
2.8944 |
1.1404 |
0.6584 |
L/D 12h |
2.6084 |
0.8381 |
0.4839 |
L/D 18h |
2.5808 |
0.4259 |
0.2459 |
L/D 24h |
2.3263 |
0.7921 |
0.4573 |
|
|
Fruiting body development |
RNA-Seq analysis of fruiting body development |
Krizsan et al. 2019
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Vegetative mycelium |
9.3651 |
1.9266 |
1.1123 |
Hyphal knots |
10.0694 |
3.4804 |
2.0094 |
Primordium 1 |
7.2944 |
1.9502 |
1.126 |
Primordium 2 |
9.1159 |
1.8369 |
1.0605 |
Young Fruiting body cap |
2.9862 |
2.7216 |
1.5713 |
Younf fruiting body gill |
7.0733 |
1.1638 |
0.6719 |
Young fruiting body stipe |
2.8173 |
0.2392 |
0.1381 |
Mature fruiting body cap_gill |
2.9983 |
0.8944 |
0.5164 |
Mature fruiting body stipe |
0.6365 |
0.2584 |
0.1492 |
|
|
Nitrogen sources (12h incubation) |
Expression profiling on diverse nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Complete medium - control |
5.4925 |
2.1026 |
1.214 |
NH4NO3 |
5.8151 |
0.2034 |
0.1175 |
Proline |
4.1154 |
1.2158 |
0.7019 |
Tryptophan |
4.7095 |
4.1308 |
2.3849 |
Isoleucin |
2.5943 |
0.1529 |
0.0883 |
Arginine |
2.0342 |
0.1823 |
0.1052 |
Metionine |
4.9711 |
0.0556 |
0.0321 |
NaNO3 |
3.4482 |
0.3772 |
0.2178 |
|
|
Plant biomass (12h incubation) |
Expression profiling on diverse plant biomasses as carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
5.4925 |
2.1026 |
1.214 |
Citrus peel |
1.5668 |
0.5218 |
0.3013 |
Poplar leaves |
5.774 |
1.1637 |
0.6719 |
Typha leaves |
4.2164 |
1.1304 |
0.6527 |
Overwintered Salix leaves |
3.7331 |
1.1738 |
0.6777 |
Reed flowering |
4.3821 |
3.5761 |
2.0646 |
Tigernut |
1.9999 |
0.6806 |
0.3929 |
Energy cane |
6.078 |
1.9143 |
1.1052 |
Guar gum |
6.2042 |
2.4261 |
1.4007 |
Apple peels |
4.2814 |
0.2562 |
0.1479 |
Cellobiose |
3.191 |
0.1992 |
0.115 |
Corn stalk |
6.7055 |
1.7244 |
0.9956 |
Horse manure |
7.1401 |
2.267 |
1.3088 |
Lignin |
2.9075 |
0.0517 |
0.0298 |
Microcrystalline cellulose |
3.5948 |
1.2701 |
0.7333 |
Oak leaves |
6.1016 |
1.0031 |
0.5791 |
Pectin esterified |
7.0906 |
1.6664 |
0.9621 |
Poplar sawdust |
8.3067 |
1.4025 |
0.8097 |
Wheat bran |
7.7228 |
0.438 |
0.2529 |
Chlamydomonas reinhardtii |
5.1166 |
1.6318 |
0.9421 |
Vertatryl alcohol |
2.575 |
0.692 |
0.3995 |
Furfural |
1.3214 |
0.3082 |
0.178 |
Autoclaved mycelium |
3.8287 |
0.4486 |
0.259 |
|
|
Simple and complex carbon and nitrogen sources |
Expression profiling on diverse complex carbon and nitrogen sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
7.5802 |
0.2667 |
0.154 |
Ribose |
8.0994 |
0.6995 |
0.4039 |
Mannose |
6.5899 |
1.5267 |
1.0795 |
Fructose |
7.2636 |
1.295 |
0.7476 |
Arabinose |
6.8329 |
0.8181 |
0.4723 |
Xylose |
8.1457 |
1.9976 |
1.1533 |
Galacturonic acid |
9.3953 |
0.9958 |
0.5749 |
Rhamnogalacturonan |
7.6945 |
0.7418 |
0.4283 |
Pectin esterified |
8.6265 |
0.5749 |
0.3319 |
Polygalacturonic acid |
9.1451 |
1.6173 |
0.9337 |
Sodium acetate |
8.5658 |
1.0008 |
0.5778 |
No nitrogen |
4.1249 |
1.4081 |
0.813 |
BSA |
7.6611 |
1.3895 |
0.8022 |
Glutamine |
6.9566 |
1.2781 |
0.7379 |
No phosphate |
5.4892 |
0.4311 |
0.2489 |
No carbon |
8.5299 |
0.6233 |
0.4407 |
No sulphur |
4.9273 |
1.3633 |
0.7871 |
|
|
Simple and complex carbon sources (12h incubation) |
Expression profiling on diverse complex carbon sources. |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose - control |
5.4925 |
2.1026 |
1.214 |
Amylose |
6.8972 |
0.7368 |
0.4254 |
Fucose |
5.8416 |
0.5193 |
0.2998 |
Galactose |
5.7483 |
0.5009 |
0.2892 |
Lactose |
6.0696 |
0.9099 |
0.5253 |
Maltose |
6.5895 |
1.5102 |
0.8719 |
Mannitol |
7.074 |
1.6494 |
0.9523 |
Rhamnose |
6.3333 |
1.3937 |
0.8047 |
Sorbitol |
7.4566 |
1.3861 |
0.8003 |
Trehalose |
6.2385 |
0.3793 |
0.219 |
Glycerol |
5.0242 |
1.349 |
0.7789 |
Glucuronic acid |
6.2115 |
0.7027 |
0.4057 |
Arabinan |
9.1504 |
2.0698 |
1.195 |
Galactan |
5.2071 |
1.6453 |
0.9499 |
Galactomannan |
7.6613 |
1.3349 |
0.7707 |
Glucan |
5.5132 |
2.9648 |
1.7117 |
Xylan |
6.6704 |
0.64 |
0.3695 |
Xyloglucan |
7.8783 |
1.4868 |
0.8584 |
Arabinogalactan |
8.5587 |
1.7217 |
0.994 |
Pectin |
14.5809 |
4.1819 |
2.4144 |
Rhamnogalacturonan |
14.3555 |
2.259 |
1.3042 |
Polygalacturonic acid |
7.2897 |
1.9282 |
1.1132 |
Mannan |
9.4835 |
2.7705 |
1.5995 |
Amylopectin |
6.9998 |
2.365 |
1.3654 |
Inulin |
4.5073 |
0.9484 |
0.5475 |
BSA |
2.5494 |
0.6435 |
0.3715 |
|
|
Starvation induced by water agar transfer |
Expression profiling on starvation induced by transfer to water agar |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Control |
1.4695 |
0.5886 |
0.3398 |
2h WAT |
2.5141 |
0.3931 |
0.227 |
4h WAT |
1.9179 |
0.8166 |
0.4715 |
8h WAT |
2.3729 |
0.5893 |
0.3402 |
16h WAT |
2.8297 |
0.7218 |
0.4167 |
24h WAT |
2.4622 |
0.8856 |
0.5113 |
WAT hyphal knot -30h |
2.0007 |
0.4731 |
0.2731 |
WAT hyphal knot -22h |
0.9128 |
0.1761 |
0.1017 |
WAT hyphal knot -15h |
2.0749 |
0.548 |
0.3164 |
WAT hyphal knot -7.5h |
3.5332 |
0.8048 |
0.4647 |
WAT hyphal knot |
2.2478 |
0.2748 |
0.1586 |
WAT hyphal knot +7.5h |
2.1549 |
1.9457 |
1.1233 |
|
|
Straw time series |
Time series expression profiling on straw as a carbon source |
Hegedus et al.
|
RNA-Seq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Glucose control |
4.0975 |
2.6797 |
1.5471 |
Straw 3h |
1.4049 |
0.4819 |
0.2782 |
Straw 6h |
2.7385 |
0.6673 |
0.3853 |
Straw 12h |
4.8697 |
1.5435 |
0.8911 |
Straw 24h |
5.6503 |
1.6911 |
0.9764 |
|
|
Stress conditions 1 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
1.1486 |
0.2983 |
0.1722 |
Frost (-20C, 30min) |
1.4442 |
0.1468 |
0.0847 |
Heat shock (47C, 2h) |
2.8216 |
0.3782 |
0.2184 |
High CO2 |
1.041 |
0.265 |
0.153 |
Drought (4% agar, 12h) |
3.3882 |
1.1914 |
0.6878 |
Oxidative (1mM H2O2, 3h) |
0.9289 |
0.0592 |
0.0342 |
Acidic (pH4, 3h) |
1.2646 |
0.8242 |
0.4758 |
Alkaline (pH9, 3h) |
1.9388 |
0.4779 |
0.2759 |
Osmotic (sorbitol 2h) |
1.6079 |
0.4503 |
0.26 |
CongoRed (3h) |
0.8075 |
0.2079 |
0.1201 |
Cobalt chloride control |
1.7942 |
0.8735 |
0.5043 |
Cobalt chloride 2mM |
1.564 |
0.8588 |
0.4958 |
|
|
Stress conditions 2 |
Expression profiling under various stress conditions |
Hegedus et al.
|
QuantSeq |
Sample |
Mean cpm |
SD cpm |
Se of mean |
Flask control |
3.8413 |
2.6146 |
1.5096 |
Cold stimulation |
2.2876 |
1.0486 |
0.6054 |
Scratched surface |
1.4327 |
0.1807 |
0.1043 |
Ca2+, 200mM, 24h |
1.1634 |
0.1465 |
0.0846 |
Cu2+, 2mM, 6h |
1.3316 |
0.2825 |
0.1631 |
Voriconazole |
1.8228 |
0.6684 |
0.3859 |
Trichoderma interaction, early |
1.879 |
1.3055 |
0.7538 |
Trichoderma interaction 60h |
1.2362 |
2.1412 |
1.2362 |
Cobalt chloride 2mM repeat |
1.4515 |
0.8992 |
0.5192 |
Hypoxia |
2.2327 |
1.2708 |
0.7337 |
Hypoxia control |
2.8333 |
1.6359 |
0.9445 |
Protoplastation control |
2.8818 |
0.2334 |
0.1651 |
Protoplastation 3.5h |
2.8015 |
0.9461 |
0.669 |
Protoplastation 1.5h |
0.6489 |
0.4992 |
0.353 |
|